Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.5.1.3 (
dihydrofolate reductase
)
5,819
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A full-length cDNA for the rat liver enzyme
tyrosine aminotransferase
has been used to construct mammalian expression vectors by recombinant DNA techniques. These vectors, which have employed either a simian virus 40 or a Rous sarcoma virus promoter, were transfected into a variety of nonhepatic mammalian cell lines in culture. Transient expression of
tyrosine aminotransferase
was readily observed after transfection into monkey COS cells and mouse L cells. Stable clones that express cloned
tyrosine aminotransferase
have been isolated from mouse L cells, hamster Wg1a fibroblasts, and Chinese hamster ovary (CHO) cells. A vector capable of expressing both
tyrosine aminotransferase
and
dihydrofolate reductase
was stimulated to undergo amplification by treatment with methotrexate in a CHO cell line deficient in the latter enzyme. Levels of
tyrosine aminotransferase
as much as 50-fold higher than typically seen in glucocorticoid-induced hepatoma cells were achieved in some CHO clones by this technique. The
tyrosine aminotransferase
produced at these highly amplified levels appeared structurally normal and had no major harmful effects on the cells.
...
PMID:Expression and amplification of cloned rat liver tyrosine aminotransferase in nonhepatic cells. 196 11
