Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.5.1.3 (
dihydrofolate reductase
)
5,819
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Two fused proteins of
dihydrofolate reductase
(
DHFR
) with oligopeptides were prepared by a recombinant DNA method. One of these,
DHFR
-IQI, has three (Ile-Gln-Ile) and the other,
DHFR
-lek, has eight (Ile-Arg-
Met-Tyr-Gly
-Gly-Phe-Leu) additional amino acid residues at the C terminals; in both proteins, Cys152 of wild
DHFR
is replaced by Glu. The thermal transition of the proteins was measured by CD and DSC at pH 7.0 and compared with that of wild
DHFR
. The results show that the thermal stability of
DHFR
-IQI is the same as that of the wild
DHFR
and that of
DHFR
-lek is less than that of the former two DHFRs. Analysis of the DSC data of
DHFR
-IQI indicates that the thermal transition is a three-state one. Data from both DSC and CD measurements suggest the association of
DHFR
-lek molecules.
...
PMID:Thermal stability of dihydrofolate reductase and its fused proteins with oligopeptides. 207 77
