Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.5.1.3 (
dihydrofolate reductase
)
5,819
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Tritium-labeled [6S]-tetrahydropteroylpolyglutamates of high radiospecific activity were prepared from the corresponding pteroylpolyglutamates.
Malic enzyme
and D,L-[2-3H]malate were used as a generating system to produce [4A-3H]NADPH which was coupled to the
dihydrofolate reductase
-catalyzed reduction of chemically prepared dihydropteroylpolyglutamate derivatives. Passage of the reaction mixtures through a column of immobilized boronate effectively removed NADPH, and the tetrahydropteroylpolyglutamates were subsequently purified by chromatography on DEAE-cellulose. Overall yields of the [6S]-tetrahydro derivatives were 18-48% and the radiospecific activities were 3-4.5 mCi X mumol-1.
...
PMID:Preparation of tritium-labeled tetrahydropteroylpolyglutamates of high specific radioactivity. 399 43
