Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.4.3.13 (
lysyl oxidase
)
1,248
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Rat aortic
lysyl oxidase
cDNA was expressed under a metallothionein promoter in Chinese hamster ovary cells using a
dihydrofolate reductase
selection marker. One methotrexate-resistant cell line, LOD-06, generated by transfecting with full-length cDNA, yielded
lysyl oxidase
proteins consistent with the 50 kDa proenzyme and a 29 kDa mature catalyst. A second cell line, LOD32-2, was generated by transfection with a truncated cDNA lacking sequences which code for the bulk of the propeptide region. Both cell lines secreted apparently identical, 29 kDa forms of mature
lysyl oxidase
each of which catalyzed the deamination of human recombinant tropoelastin and alkylamines, consistent with the known specificity of
lysyl oxidase
. The secreted enzyme forms were inhibited by chemical inhibitors of
lysyl oxidase
activity, including beta-aminopropionitrile, phenylhydrazine, ethylenediamine, alpha, alpha'-dipyridyl, and diethyldithiocarbamate. Sensitivity to these agents is consistent with the presence of copper and carbonyl cofactors in the expressed enzymes, characteristic of
lysyl oxidase
from connective tissues. These results indicate the lack of essentiality of the deleted proprotein sequence for the proper folding, generation of catalytic function, and secretion of
lysyl oxidase
.
...
PMID:Expression of lysyl oxidase from cDNA constructs in mammalian cells: the propeptide region is not essential to the folding and secretion of the functional enzyme. 856 51
