Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.4.3.11 (glutamate dehydrogenase)
 4,437 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A sequential injection analysis (SIA) is proposed for the simultaneous determination of L- and D-methotrexate (Mtx) using amperometric biosensors as detectors. A SIA system is proposed due to the highest precision and accuracy and lower consumption of sample and buffer. The amperometric biosensors used as detectors in SIA system were based on L-amino acid oxidase (L-AAOD) or/and L-glutamate oxidase (L-Glox) and horseradish peroxidase (HRP) for the assay of L-Mtx and D-amino acid oxidase (D-AAOD) and HRP for the assay of D-Mtx were selected. The linear concentration ranges are of pmol/l to nmol/l magnitude order, with very low limits of detection. The SIA/biosensors system can be used reliably on-line in synthesis process control, for the simultaneous assay of L- and D-Mtx with a frequency of 34 samples per hour.
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PMID:Simultaneous determination of L- and D-methotrexate using a sequential injection analysis/amperometric biosensors system. 1461 62

In order to determine the enantiopurity of methotrexate (Mtx), seven biosensors were proposed for the assay of L-Mtx and three biosensors for the assay of D-Mtx. The biosensors were designed using physical and chemical immobilization of glutamate oxidase and/or L-amino acid oxidase (L-AAOD) and/or horseradish peroxidase (HRP) for the assay of L-methotherexate, and D-amino acid oxidase (D-AAOD) and HRP for the assay of D-Mtx. Electrode characteristics were obtained and compared for the different carbon paste based biosensors. The linear concentration ranges for the proposed biosensors were in the ranges of fmol l(-1) to pmol l(-1), magnitude order with limits of detection in the fmol l(-1) to nmol l(-1) concentration range. All biosensors were successful for the determination of the enantiopurity of Mtx as raw material, and in its pharmaceutical formulations (tablets and injections).
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PMID:Determination of L- and D-enantiomers of methotrexate using amperometric biosensors. 1896 24


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