Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.4.3.11 (glutamate dehydrogenase)
 4,437 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Primary roots of soybean [Glycine max (L.), cv Harosoy 63] seedlings were inoculated with zoospores from either race 1 (incompatible, host resistant) or race 3 (compatible, host susceptible) of Phytophthora megasperma f. sp. glycinea (Pmg) and the activities of phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), isoflavone synthase, and dihydroxypterocarpan 6a-hydroxylase related to phytoalexin (glyceollin) biosynthesis, and of glucose-6-phosphate dehydrogenase (Glc-6-PDH) and glutamate dehydrogenase (Glu-DH) were determined at various times after inoculation. About 2-4 h after inoculation with race 1, the activities of PAL, CHS, and pterocarpan 6a-hydroxylase were higher than after inoculation with race 3 and increased considerably thereafter. In contrast, activities of these enzymes in the compatible interaction were equal to or only slightly higher than in the controls over the entire infection period investigated (2-8 h). Isoflavone synthase did not increase until 7 h after inoculation with race 1. There were no significant differences in activities for Glc-6-PDH and Glu-DH between inoculated roots and controls. The results show that infection of soybean roots with zoospores of Pmg race 1 causes a race:cultivar-specific early induction of enzymes involved in glyceollin synthesis, whereas such an induction does not occur with zoospores of race 3. These findings are in agreement with the race:cultivar-specific accumulation of glyceollin in soybean roots reported previously [M. G. Hahn, A. Bonhoff, and H. Grisebach (1985) Plant Physiol. 77, 591-601].
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PMID:Race:cultivar-specific induction of enzymes related to phytoalexin biosynthesis in soybean roots following infection with Phytophthora megasperma f. sp. glycinea. 396 19

Treatment of cell suspension cultures of Phaseolus vulgaris c.v. Immuna with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum resulted in rapid accumulation of the prenylated 5-hydroxyisoflavanone phytoalexin kievitone followed by later accumulation of the pterocarpan-derived phytoalexin phaseollin. Kievitone formation was preceded by rapid transient increases in the extractable activities of the enzymes L-phenylalanine ammonia-lyase and chalcone synthase. The extractable activities of 15 enzymes were measured in the cell cultures during the period of kievitone accumulation. The results suggest a highly selective induction of enzymes associated directly with the phytoalexin pathway. No induction of enzymes of pathways diverging from or providing substrates for the phenylpropanoid----isoflavonoid pathway was observed. The increase in glutamate dehydrogenase activity in control cultures was prevented by elicitor application. A comparison of enzyme activities in control and Colletotrichum-infected bean hypocotyls provided further evidence of the selective induction of enzymes of phytoalexin synthesis, although peroxidase, glutamate dehydrogenase and glutamate synthase activities were higher in infected than in healthy hypocotyls. It is concluded that the major enzymic changes occurring in elicitor-treated bean cells are probably those directly associated with defence mechanisms such as the formation of isoflavonoid phytoalexins (this paper) or accumulation of phenolic compounds and hydroxyproline-protein in the cell walls [Bolwell, G. P. et al. (1985) Eur. J. Biochem. 148, 571-578].
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PMID:Metabolic changes in elicitor-treated bean cells. Selectivity of enzyme induction in relation to phytoalexin accumulation. 399 94