Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.4.1.2 (
glutamate dehydrogenase
)
4,380
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Synchrony of energy and nitrogen release in rumen has been proposed to maximize ruminal microbial fermentation. However, the information regarding bacterial community composition and its metabolism under a higher or lower degree of synchronization is limited. In our study, a 0 to 6 h post-feeding infusion (first half infusion,
FHI
), 6 to 12 h post-feeding infusion (second half infusion, SHI), and 0 to 12 h post-feeding infusion (continuous infusion, CI) of maltodextrin were used to simulate varying degrees of synchronization of energy and nitrogen release in a rumen simulation system. In addition, the bacterial community, metabolite, enzyme activity, and microbial protein synthesis (MPS) were evaluated. Compared with the
FHI
and CI, the relative abundance of
Fibrobacter
,
Ruminobacter
, BF311, and CF231 decreased in the SHI, but that of
Klebsiella
and
Succinivibrio
increased in the SHI. The NH
3
-N and branched-chain volatile fatty acids were significantly higher, but propionate content and activities of
glutamate dehydrogenase
(
GDH
) and alanine dehydrogenase were significantly lower in the SHI than those in the
FHI
and CI. The SHI had lower MPS and less efficiency of MPS than the
FHI
and CI, which indicated that the SHI had a lower degree of synchronization. Correlation analysis showed that MPS was positively related to
GDH
activity and relative abundance of
Fibrobacter
but negatively related to NH
3
-N and relative abundance of
Klebsiella
. Therefore, a higher degree of synchronization of energy and nitrogen release increased MPS partly via influencing the bacterial community, metabolism, and enzyme activities of ammonia assimilation in the in vitro fermenters.
...
PMID:Synchrony Degree of Dietary Energy and Nitrogen Release Influences Microbial Community, Fermentation, and Protein Synthesis in a Rumen Simulation System. 3205 Apr 6
