Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.4.1.2 (
glutamate dehydrogenase
)
4,380
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The interaction between n-
octyl
-beta-D-glucopyranoside (
octyl
glucoside) and bovine liver
glutamate dehydrogenase
(
GDH
) was studied using techniques including equilibrium dialysis, UV-spectrophotometry, circular dichroism (CD), fluorescence energy transfer and extrinsic spectrofluorometry in 50 mM sodium phosphate buffer solution (pH 7.6). The equilibrium dialysis experiment showed a higher binding of
octyl
glucoside to
GDH
that induces up to 80% enzyme inhibition in 20 mM
octyl
glucoside solution. The CD study indicated that
GDH
retains its secondary structure in the presence of
octyl
glucoside, but loses a degree of its tertiary structure by acquiring a more extended tertiary structure. Measurement of the binding of a hydrophobic fluorescent probe, 1-anilino-naphthalene-8-sulfonate (ANS), to
GDH
revealed that the binding of ANS to
GDH
is increased in the presence of
octyl
glucoside, a finding that may be interpreted in terms of the increment of surface hydrophobic patch(es) of
GDH
because of its binding to
octyl
glucoside. Fluorescence energy transfer studies also showed more binding of the reduced coenzyme (NADH) to
GDH
and the Lineweaver-Burk plots (with respect to NADH) indicate the existence of substrate inhibition in the presence of
octyl
glucoside. These observations are aimed at explaining the formation of the molten globule-like structure of
GDH
, which is induced by a non-ionic detergent such as
octyl
glucoside.
...
PMID:Octyl glucoside induced formation of the molten globule-like state of glutamate dehydrogenase. 1168 30
