Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.4.1.2 (glutamate dehydrogenase)
 4,380 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The method of fluorescent titration was used to study the effect of carbonic acid on the process of NADH binding with the purified preparation of glutamate dehydrogenase as well as on fluorescent properties of the above mentioned coenzyme. It is shown that in bicarbonate buffer at the alkaline values of pH there is a decrease in the maximal capacity of the binding sites of the enzyme with low affinity to co-enzyme. The capacity of high-affinity binding sites in this case is unchanged. It is found that pCO2, HCO3- and H+ exert an effect on the dissociation constants of the NADH-glutamate dehydrogenase complex as well as on fluorescent properties of bound NADH. It is supposed that the effects observed are a result of the interaction of dissolved carbon dioxide with free amino groups of protein molecule.
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PMID:[Effect of carbonic acid on glutamate dehydrogenase interaction with NADH]. 339 78

The initial rate of direct and reverse reactions catalyzed by crystalline NAD(P)+-dependent glutamate dehydrogenase from bovine liver as well as Km, V and some other kinetic parameters were studied as affected by carbonic acid in different concentrations (0, 10, 20, 40, 60 mM) at p Hof the incubation media 6.4-9.1. It is shown that the dependence of the initial rate on pH both of the direct and reverse reactions varies with the carbonic acid concentration. In the pH alkaline range the glutamate dehydrogenase activity is inhibited in the presence of a mixture of HCO3 and pCO2 (soluble carbonic acid) and in the acid range pH it has a tendency to increase. The pKb value lowers by an order and the pKa value remains practically unchanged. The interaction of pCO2 with E-amino group of the lysin residue in the enzyme molecule accompanied by formation of carbamate is supposed to be possible. The bicarbonate ion may also affect the enzyme.
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PMID:[Effect of different levels of carbonic acid on glutamate dehydrogenase activity]. 681 28