Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.4.1.2 (glutamate dehydrogenase)
 4,380 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

125I-N6-(N-[6-N-(5-iodo-4-azidosalicyl)-aminohexyl]- aminocarbamoylmethyl)-nicotinamide adenine dinucleotide (125I-N6-I-ASA-AH-NAD+) was synthesized by coupling N6-([6-aminohexyl]-carbamoylmethyl)-NAD+ with 4-azidosalicylic acid N-hydroxysuccinimide ester followed by radioiodination. The utility of 125I-N6-I-ASA-AH-NAD+ as an effective site-directed photoprobe was demonstrated by the photolabeling of both glutamate dehydrogenase and 15-hydroxyprostaglandin dehydrogenase. Both enzymes can be saturated with labeled probe with apparent dissociation constants comparable to those reported for NAD+. Photoincorporation of the probe into both enzymes was found to be protected specifically by NAD+. These results indicate that 125I-N6-I-ASA-AH-NAD+ can be a specific photoprobe for NAD(+)-linked enzymes.
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PMID:An 125I-labeled N6-substituted azido analog of NAD+ for the photoaffinity labeling of NAD(+)-linked enzymes. 780 Jul 17

Individual risk factors for clinical mastitis within the first month of lactation (early clinical mastitis, ECM) were analyzed in 695 Holstein multiparous cows (561 ECM- and 134 ECM+) which were clinical mastitis-free during the previous lactation. They belonged to 44 herds from the French Brittany region and were surveyed for 4 years. Individual biological parameter levels during the last 2 months of gestation, production of the preceding lactation and calving conditions and health were the available explanatory variables. The construction and explanation model used multiple logistic regression run with herd as a fixed effect. High precalving plasma ceruloplasmin oxidase (p < 0.01) and low glutamate dehydrogenase (p < 0.05) activities, high 305-day previous-lactation milk yield (p < 0.001) and milk protein content at the last milk-test day before drying-off (p < 0.01) were the significant risk factors for ECM occurrence. Intensive production and unsatisfactory dietary conditions (energy and antioxidants) would increase ECM risk in the dairy cow. Further studies should be conducted to determine if metabolic and/or genetic factors could explain the relationship between ceruloplasmin and subsequent ECM occurrence.
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PMID:Biological predictors for early clinical mastitis occurrence in Holstein cows under field conditions in France. 963 78