Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.4.1.2 (glutamate dehydrogenase)
 4,380 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We have developed soluble extracts from Neurospora crassa capable of accurately initiating the transcription of cloned Neurospora protein-encoding genes by RNA polymerase II in vitro. The genes encoding glutamate dehydrogenase (am) and histones H3 and H4 were transcribed by the extracts, and transcription was sensitive to alpha-amanitin at 1 mg/ml. The 5' heterogeneity of the in vitro initiation reactions was highly specific. Of the 17 transcription initiation sites within the inducible qa gene cluster, only one minor site was used in vitro, suggesting that, in general, transcription from qa gene promoters requires at least one different protein from those required for transcription of the am and histone genes.
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PMID:Accurate transcription of cloned Neurospora RNA polymerase II-dependent genes in vitro by homologous soluble extracts. 299 27

RNA polymerase from the hyperthermophile archaeon Pyrococcus furiosus (Pfu) forms specific and transcriptionally active complexes with its conjugate transcription factors TBP (the archaeal TATA binding protein homolog) and TFB (the archaeal homolog of eukaryotic RNA polymerase II and III transcription factors TFIIB and Brf) at the Pfu glutamate dehydrogenase promoter. A photochemical crosslinking method was used to map the vicinity of the catalytic subunits of Pfu RNA polymerase to DNA locations distributed along the polymerase-promoter interface. The largest component of this archaeal polymerase is split into two subunits, A' and A", whose relatively sharp boundary of DNA crosslinking (probed on the transcribed strand) is centered five to six base pairs downstream of the transcriptional start site. A strong argument based on this information, on the well-defined homology between the core bacterial, archaeal and eukaryotic RNA polymerase subunits, and on the recently determined structure of a bacterial RNA polymerase specifies the directionality of DNA in the archaeal transcription complex and its trajectory downstream of the transcriptional start site.
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PMID:The orientation of DNA in an archaeal transcription initiation complex. 1096 30