Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.4.1.2 (
glutamate dehydrogenase
)
4,380
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In order to gain insight into the metabolic modifications induced in rat brain tissues by helium-neon (He-Ne) laser irradiation, in the research described here, we investigated the variations in the activity of the enzymes aspartate transferase (AST,
EC 2.6.1.4
), both cytosolic and mitochondrial,
glutamate dehydrogenase
(GIDH, EC 1.4.1.3), and total superoxide dismutase (SOD, EC 1.15.1.1), in the brain of rats treated with a very small dose (1.08 J) of He-Ne laser radiation. The rats were sacrificed 4 h after the treatment. The enzymes were evaluated spectrophotometrically in brain extracts of irradiated animals and also in untreated rats (controls) and rats that underwent simulated treatment (stressed). The data obtained from 5-10 animals assayed individually showed that, in the in toto brain tissues of the irradiated rats compared to the stressed rats, there was a marked increase of total SOD, together with an appreciable decrease of cytosolic AST, and insignificant variations in mitochondrial AST and GIDH. Stress alone caused a considerable decrease of total SOD and small but statistically significant increases of s-AST, m-AST, and GIDH.
...
PMID:Rat brain metabolism enzyme activity variations following He-Ne laser irradiation. 177 92
The following enzymatic activities were demonstrated in Myxococcus xanthus during growth and myxospore formation: l-alanine dehydrogenase (EC 1.4.1.1), l-
glutamic acid dehydrogenase
(
EC 1.4.1.2
), glycine dehydrogenase (EC 1.4.1.5), l-glutamic glyoxylate aminotransferase (
EC 2.6.1.4
), and l-alanine glyoxylate aminotransferase (EC 2.6.1.12).
...
PMID:Presence of amino acid dehydrogenases and transaminases in Myxococcus xanthus during vegetative growth and myxospore formation. 421 65
As glutamate and ammonia play a pivotal role in nitrogen homeostasis, their production is mediated by various enzymes that are widespread in living organisms. Here, we report on an effective electrophoretic method to monitor these enzymes. The in gel activity visualization is based on the interaction of the products, glutamate and ammonia, with
glutamate dehydrogenase
(GDH, EC: 1.4.1.2) in the presence of either phenazine methosulfate (PMS) or 2,6-dichloroindophenol (DCIP) and iodonitrotetrazolium (INT). The intensity of the activity bands was dependent on the amount of proteins loaded, the incubation time and the concentration of the respective substrates. The following enzymes were readily identified: glutaminase (EC: 3.5.1.2), alanine transaminase (EC: 2.6.1.2), aspartate transaminase (EC: 2.6.1.1),
glycine transaminase
(EC: 2.6.1.4), ornithine oxoacid aminotransferase (EC: 2.6.1.13), and carbamoyl phosphate synthase I (EC: 6.3.4.16). The specificity of the activity band was confirmed by high pressure liquid chromatography (HPLC) following incubation of the excised band with the corresponding substrates. These bands are amenable to further molecular characterization by a variety of analytical methods. This electrophoretic technology provides a powerful tool to screen these enzymes that contribute to nitrogen homeostasis in Pseudomonas fluorescens and possibly in other microbial systems.
...
PMID:A blue native polyacrylamide gel electrophoretic technology to probe the functional proteomics mediating nitrogen homeostasis in Pseudomonas fluorescens. 2259 84
