Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.4.1.2 (
glutamate dehydrogenase
)
4,380
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
This work has looked to explore an innovative and powerful visible fluorescence immunoassay method through wet NH
3
-triggered structural change of NH
2
-
MIL
-125(Ti) impregnated on paper for the detection of carcinoembryonic antigen (CEA). Gold nanoparticles heavily functionalized with
glutamate dehydrogenase
(
GDH
) and secondary antibody were used for generation of wet NH
3
with a sandwiched immunoassay format. Paper-based analytical device (PAD) coated with NH
2
-
MIL
-125(Ti) exhibited good visible fluorescence intensity through wet NH
3
-triggeried structural change with high accuracy and reproducibility. Moreover, NH
2
-
MIL
-125(Ti)-based PAD displayed two visual modes of fluorescence color and physical color with the naked eye and allowed the detection of CEA at a concentration as low as 0.041 ng mL
-1
. Importantly, the PAD-based assay provides promise for use in the mass production of miniaturized devices and opens new opportunities for protein diagnostics and biosecurity.
...
PMID:Wet NH
3
-Triggered NH
2
-MIL-125(Ti) Structural Switch for Visible Fluorescence Immunoassay Impregnated on Paper. 3046 75
