Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.3.5.1 (succinate dehydrogenase)
 8,177 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. No essential differences were found in the activities of tricarboxylic acid-cycle enzymes in the newly isolated facultative methylotroph Pseudomonas J26 and obligate methylotroph Methylomonas Pl1. 2-Oxoglutarate dehydrogenase and succinate dehydrogenase were absent in Methylomonas Pl1; in Pseudomonas J26 the functioning of the cycle was imparied only on the methanol medium. Citrate synthase of both organisms showed low sensitivity to 2-oxoglutarate, NADH and ATP. 2. In both methylotrophs, methanol dehydrogenase was inhibited non-competitively by ATP: the activity was reduced by half by ATP at a concentration of 5 mM. 3. Concentration of ATP in the log-phase cultures of Methylomonas Pl1 was about twice as high as in Pseudomonas J26 (4.7 and 1.7 mumol/g dry wt., respectively). 4. Differences between the energy state of Methylomonas Pl1 and Pseudomonas J26 might be due to the higher ability of the former to oxidize methanol and/or lower energy requirement for C1 assimilation by the hexulose pathway in the obligate methylotroph.
Acta Biochim Pol 1979
PMID:Tricarboxylic acid-cycle enzymes and ATP pool in facultative and obligate methylotrophs: Pseudomonas J26 and Methylomonas Pl1. 12 Oct 7

Histopathological, histochemical and biochemical investigations were performed on the brain, sciatic nerve, skeletal muscle, heart, liver and kidney of rats which were given 5% of LD50 dose of DFP for 10 days. A decrease in AChE activity, degeneration of neurons and necrotic changes in the nuclei of hypothalamus, degeneration of myelin sheaths in sciatic nerve, a decrease in succinic dehydrogenase activity in the myocardium, and a minimal decrease of acid phosphatase activity (AcPh) in the liver were found. The biochemical determination of AChE level indicated about 30% AChE activity in erythrocytes and tibialis muscle, and 40% in the brain 1 hr after the last dose of the inhibitor and 80% and 50% respectively on the 7th day after poisoning in relation to normal values.
Acta Physiol Pol
PMID:Histopathology, histochemistry and acetylcholinesterase activity after repetitive administration of fluostigmine to albino rat. 53 68

Phenytoin was asministered to rats per os in the dose 110 mg/kg for 6 months in order to check its effect on cerebellar succinic dehydrogenase activity. The latter appeared to be unchanged, even in those cerebellar structures which showed features of alteration.
Pol Med Sci Hist Bull
PMID:Effect of phenytoin on succinic dehydrogenase activity in rat cerebellum. 122 4

Rats kept on a low-magnesium diet for 14 weeks developed increasing clinical symptoms, from slight trophic changes to inhibition of growth. Focal fragmentation of myocardial fibers was followed by focal fuchsinophilia and later--by focal necrosis. Increasing morphological lesions were associated with calcium salts deposition in the degenerated myocardial fibers, connective tissue and wall of cardiac blood vessels. The low-magnesium diet did not affect the activity of ATP-ases and cytochrome oxidase. The areas of myocardial degeneration and necrosis were free of the activity of alkaline phosphatase and succinic dehydrogenase.
Pol Med Sci Hist Bull
PMID:Effect of low-magnesium diet on the histology and the activity of certain enzymes of rat myocardium. 122 2

The protein content and activity of enzymatic markers of cell organelles: succinate dehydrogenase, glucose-6-phosphatase, uricase, acid phosphatase, 5'-nucleotidase and alkaline phosphatase were assayed in the homogenate and the supernatant (after two-hour centrifugation at 140,000 X g) of the liver and intestinal epithelium in rabbits irradiated with a single dose of 550 rads of gamma rays. The determinations were carried out on 1,3,6,9,15 and 30 days after irradiation for experimental and control animals. After gamma irradiation the following alterations were found: 1) increase in protein content (marked between 3-6 days), 2) remarkable rise of alkaline phosphatase activity (during the entire period of study), 3) elevation of 5'-nucleotidase activity (only in the intestinal epithelium), 4) marked reduction of succinate dehydrogenase and uricase activity (on the first day of study), 5) moderate decrease of glucose-6-phosphatase activity (mainly on the third day). Apart from a slight decline in the activity of acid phosphatase in the homogenate of intestinal epithelium, on the third day there practically were no changes in the activity of this enzyme either in the supernatant of intestinal epithelium or in the liver tissue.
Pol Med Sci Hist Bull
PMID:Effect of gamma radiation on the enzymatic activity of cell organelles of liver and epithelium of small intestine in rabbits. 123 88

The experiments carried out for the study aimed at determining the prenatal sensitivity to cadmium in the aspect of beth morphological and functional condition of placenta. The experiments were carried out on 80 pregnant female rats divided into three experimental groups and a control one. The experimental animals were daily administered, intragastrically, aqueous solution of cadmium chloride, from the 7th to the 19th day of pregnancy, in doses differing between the experimental groups: 2, 10 and 22 mg of CdCl2 per kilogram of body weight, respectively. The animals were decapitated on the 21st day of pregnancy. Placenta sections, besides hematoxylin and eosin staining, were examined for the activity of the following enzymes: succinic dehydrogenase (SDH) E.C.1.3.99.1., lactate dehydrogenase (LDH) E.C.1.1.1.27., NADH-tetrazole reductase (NADH-t.r.), without a catalog number, and their glycogen reaction was checked. The results of the experiments proved cadmium chloride to cause considerable damage to the enzymatic apparatus of placenta, leading to energy deficit in the cells of both the fetal and the maternal part of this organ. The multienzymatic changes observed preceded the morphologic ones by far.
Ginekol Pol 1992 Jun
PMID:[Dynamics of morphological and cytochemical changes in the placenta following cadmium chloride intoxication]. 130 26

Cyclophosphamide administered intraperitoneally in 20 mg/kg doses at intervals of six days for six weeks caused focal morphological and histochemical lesions of low intensity in rat myocardium. From the third week of the experiment focal eosinophilia and fuchsinophilia of the cytoplasm of a part and later on, of the whole cytoplasm of myocardial fibres, contraction nodes, undulant shape of the fibres, rupture of intercalated discs, very rarely in the sixth week small focal infiltrations composed of mononuclear cells at the site of damaged and disintegrated myocardial fibres, small amount of glycogen, focal increase of acid phosphatase activity and low-grade loss of alkaline phosphatase and magnesium and calcium-dependent ATPases in the sixth week, and unchanged activity of succinic dehydrogenase. Simultaneous use of cyclophosphamide and low-magnesium diet increased the intensity of degenerative changes, which appeared from the second week on, and necrotic changes appearing from the third week on, with more pronounced disturbances in the activity of all studied enzymes, including succinic dehydrogenase, in the sixth week.
Mater Med Pol
PMID:Histological and histochemical examinations of the myocardium of rats kept on low-magnesium diet and treated with cyclophosphamide. 213 21

Methotrexate administration in 1 mg/kg doses intraperitoneally twice weekly for six weeks caused in rat myocardium the following focal, not very intense, histological and histochemical changes from the third week of the experiment on: focal increasing eosinophilia and fuchsinophilia of myocyte cytoplasm, undulating shape of myocardial fibres, contraction nodes, in the sixth week small necrotic foci with associated infiltrations composed of mononuclear cells, presence of p.a.S. positive substance resistant to diastase digestion in foci of damaged cardiomyocytes, focal rise of acid phosphatase, local loss of calcium and magnesium dependent ATPases in the sixth week, and unchanged succinic dehydrogenase activity. Simultaneous administration of low-magnesium diet and methotrexate caused more frequent (from the second week on) the appearance of more intense degenerative changes, and from the third week on the appearance of necrotic changes of cardiomyocytes, local increase of acid phosphatase activity from the second week on, falling activity of calcium and magnesium dependent ATPases from the fifth week on, and losses of succinic dehydrogenase activity in the sixth week of the experiment.
Mater Med Pol
PMID:Histological and histochemical examinations of myocardium of rats kept on low-magnesium diet and receiving methotrexate. 215 93

Rat kidney was studied histochemically and in electron microscope during administration of lithium carbonate for 14 days in doses of 4 mmol/l. Morphological examination demonstrated signs of damage exclusively to the epithelial cells in the proximal tubule. Histochemical examination demonstrated a major reduction of the reactions for succinate dehydrogenase and cytochrome oxidase. No difference was found in the intensity of the reaction for alkaline phosphatase and Ca-ATPase during lithium treatment as compared to controls. Additional observation demonstrated, only in histological examination, an increased number of cells of the macula densa.
Mater Med Pol
PMID:Ultrastructural and histochemical assessment of proximal renal tubule in rats during administration of lithium carbonate. 251 37

The aim of this study was to determine activities of selected enzymes, i.e. Mg(++)-stimulated ATP-ase, acid phosphatase (AcP), esterase and succinate dehydrogenase (SDH) in rat liver after intragastric administration of cadmium chloride. The investigations were performed on forty male rats, Wistar strain, which made up one control group and two experimental groups. The rats of one experimental group were given a single dose of cadmium chloride solution of 88 mg CdCl2 per kg body weight (Group I), whereas the other experimental group received a double dose, i.e. 176 mg CdCl2 per kg body weight (Group II). The activities of enzymatic reactions were determined by means of analysis of surface optical density using the image computer analyzer, whereas the degree of mental accumulation was estimated with atom absorption spectrophotometry. Following cadmium chloride administration, the activities of SDH and esterase decreased in both experimental groups compared to controls. The decrease in both enzymatic activities was statistically significant. Similarly, activities of ACP and Mg(++)-stimulated ATP-ase decreased below control level in the rats of the Group I, which were given lower dose of CdCl2. This change was not statistically significant. Higher dose of CdCl2 (Group II) caused a statistically significant increase in acid phosphatase activity and only a small increase (bearing no statistical significance) in Mg(++)-stimulated ATP-ase activity. In addition, it was found that the accumulation of cadmium in both the liver and the kidneys is dose-related.
Pol J Occup Med 1989
PMID:Effect of cadmium upon the activity of some selected marker enzymes in rat liver. 253 52


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