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Query: EC:1.3.5.1 (succinate dehydrogenase)
 8,177 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

From a Bacillus subtilis gene bank constructed in Escherichia coli and based on a low copy number cloning vector we have isolated a hybrid plasmid, pSH1047, containing an 8.0 kb segment of B. subtilis DNA coding for the sdhA, B and C genes, which code for the component polypeptides of succinate dehydrogenase, and the gerE gene, which may code for or regulate a protease involved in producing spores which germinate normally. We report the restriction map of this segment and the analysis of deletion derivatives which allow us to correlate the physical and genetic maps of these chromosomal segments.
J Gen Microbiol 1985 Sep
PMID:Cloning and deletion analysis of a genomic segment of Bacillus subtilis coding for the sdhA, B, C (succinate dehydrogenase) and gerE (spore germination) loci. 393 34

We have shown that the low histidase activity found in anaerobic, nitrogen-limited cultures of Klebsiella pneumoniae is due to repression of the right-hand hut operon. In addition, we have examined the effects of NO3- on the aerobic and anaerobic expression of catabolite- and NH4+-repressible enzymes in this organism. NO3- permitted anaerobic growth of K. pneumoniae in minimal medium containing histidine as the sole carbon source, and histidase and succinate dehydrogenase were derepressed during anaerobic growth in histidine/NO3- medium. Use of sucrose rather than histidine as the carbon source reversed the effects of NO3- and repressed histidase and succinate dehydrogenase activities. Anaerobic growth in sucrose/NO3- medium also uncoupled the expression of urease and glutamine synthetase.
J Gen Microbiol 1982 Jul
PMID:Effects of anaerobiosis and nitrate on the expression of succinate dehydrogenase and enzymes associated with nitrogen metabolism in Klebsiella pneumoniae. 612 18

Phages capable of transducing succinate dehydrogenase mutants (sdh) of Escherichia coli were isolated from pools of artificially constructed recombinant lambda phages using a selective casein digest medium. These phages produced characteristically dense turbid plaques, and as prophages they increased the aerobic growth efficiencies of sdh mutants on complex media but were unable to promote growth with succinate as sole carbon and energy source (an essential feature of sdh + strains). The phages were identified as fumarate reductase transducing phages (lambda frdA) by the presence of a characteristic 4.9 kilobase pairs R.HindIII fragment of bacterial DNA, the expression of a polypeptide with a relative molecular mass of 72,000 (the frdA gene product) and by comparing their transducing activities with authentic lambda frdA phages. In parallel studies a strain containing a ColE1-frd hybrid plasmid (pGS1 = pLC16.43) was characterized. Transfer of pGS1 to sdh mutants was accompanied by increased aerobic growth efficiencies on complex media and the ability to utilize succinate as sole carbon and energy source. It was concluded that fumarate reductase can replace succinate dehydrogenase but the extent of the reversal of the sdh lesion depends on frd gene dosage and the titration of the repressor which normally prevents aerobic synthesis of the reductase.
J Gen Microbiol 1981 Feb
PMID:Partial replacement of succinate dehydrogenase function by phage- and plasmid-specified fumarate reductase in Escherichia coli. 627 99

The Clarke-Carbon colony bank containing ColE1-Escherichia coli hybrid plasmids was screened by conjugation for complementation of the citrate synthase lesion of a gltA mutant. Three ColE1-gltA+ plasmids were identified: pLC26-17 (16.3 kilobase pairs), pLC27-18 (16.35 kb) and pLC31-28 (26.0 kb). The citrate synthase activities of strains containing the hybrid plasmids were amplified 3- to 10-fold. Genetic studies indicated that the smaller plasmids may contain at least part of the succinate dehydrogenase gene (sdh). A physical map of a 19.4 kb region of the E. coli chromosome containing the citrate synthase gene (gltA) was constructed by restriction analysis with the isolated plasmids. The relative positions of 20 restriction sites were defined and a region (3.1 kb) containing the gltA+ gene was identified in the segment common to all three plasmids.
J Gen Microbiol 1981 May
PMID:Hybrid plasmids containing the citrate synthase gene (gltA) of Escherichia coli K12. 627 5

The acids produced in broth culture by various species of oral haemophili and by stock strains of capsulated and other haemophili were identified and measured by gas-liquid chromatography. Succinic acid was the major acid end-product of all strains, with acetic acid also being regularly produced but in smaller amounts. A stock strain, Haemophilus parainfluenzae NCTC 4101, produced less succinic acid than other strains of haemophili. Strain NCTC 4101 possessed all the enzymes of the tricarboxylic acid cycle, as previously reported, but in the other haemophili examined only succinic dehydrogenase, fumarase and malate dehydrogenase could be detected. No other enzymes of the tricarboxylic acid cycle were detected and isocitrate lyase, malate synthase and pyruvate carboxylase were also absent. Phosphoenolpyruvate-carboxylase was present in all strains. A partial tricarboxylic acid cycle and marked malate dehydrogenase activity appear to be characteristic of haemophili. The pathway to succinate in haemophili appears to be via carboxylation of phosphoenolpyruvate to oxalacetate and thence via malate and fumarate. The results of tracer studies on a single oral strain of H. parainfluenzae using various labelled substrates were in keeping with this proposed metabolic pathway.
J Gen Microbiol 1984 Jul
PMID:The acid end-products of glucose metabolism of oral and other haemophili. 633 75

A succinate dehydrogenase-negative mutant of Bacillus subtilis is described which lacks all three subunits of the membrane-bound succinate dehydrogenase complex: flavoprotein, iron protein, and cytochrome b558. The corresponding mutation is revertible and it maps at one extreme of the sdh region. The results presented suggest that the structural genes for the subunits of the succinate dehydrogenase complex are part of one operon.
J Gen Microbiol 1983 Apr
PMID:Characterization of a pleiotropic succinate dehydrogenase-negative mutant of Bacillus subtilis. 641 59

The brain of the fishes (Channa punctatus) subjected to cold acclimation (15 +/- 1 degree) in darkness exhibited low succinic dehydrogenase (SDH) activity after 3 and 7 days and high protein content after 7 days when compared with their warm acclimated (32 +/- 1 degree) counterparts. In warm acclimated fishes maintained in complete darkness, T4 (0.5 micrograms/g body weight/day) depressed the enzyme activity after 5 and 7 days of treatment and reduced the protein content after 3 days. But neither in cold acclimated fishes maintained in darkness for 3, 5, and 7 days nor in warm acclimated fishes maintained in 12 hr dark and 12 hr daylight for the same periods did T4 induce a significant change in the same biochemical parameters. It appears that T4 action in this fish is dependent on acclimation temperature and light:dark regimes. When warm acclimated control fishes maintained in complete darkness were compared with those maintained in 12 hr dark and 12 hr daylight, the enzyme activity was found to be higher and the protein content lower in the former than in the latter. The results suggest that natural photoperiod regulates the thyroid activity in vivo. In vitro studies revealed that the presence of T4 (3.12 microM) in the incubating medium stimulated the enzyme activity of brain homogenates possibly due to direct action on mitochondria. Immersion of fishes in thiourea solution (1 mg/ml) for 3, 5, and 7 days resulted in enhancement of enzyme activity after 7 days of treatment and of protein content after 5 days of treatment. Thyroid hormones in vivo appear to have an inhibitory effect on the carbohydrate metabolism of the nervous tissue.
Gen Comp Endocrinol 1984 Mar
PMID:Thyroid hormones and carbohydrate metabolism of brain in the teleost, Channa punctatus. I. Effect of T4 and thiourea on succinic dehydrogenase (SDH) activity and protein content. 642 14

Two types of fumarate reductase transducing phages, lambda frdA, carrying the wild-type frdA gene but differing in the orientation of a R.HindIII fragment of bacterial DNA were isolated from populations of recombinant transducing phages by their ability to complement the lesions of frdA mutants of E. coli. In lysogens, the cloned frdA gene was controlled by its own promoter and was fully responsive to normal regulatory stimuli. The lambda frdA phages would not complement the defects of succinate dehydrogenase (sdh) mutants. Genetic studies showed that the R.HindIII fragment contains ampA, the cis-acting regulatory locus for the chromosomal beta-lactamase gene ampC. No evidence for the presence of other markers was detected but the bacterial segment could be extended to produce plaque-forming phage derivatives containing the amp operon and a gene concerned with bacteriophage morphogenesis, groE(mop). A physical map of the 4.9 kb R.HindIII fragment was constructed by restriction analysis and flanking fragments were identified by DNA:DNA hybridization analysis. The frdA region contained a single asymmetric R.EcoRI target 3.33 kb from one end and the orientation of the physical map with respect to the E.coli linkage map was established.
Mol Gen Genet 1980
PMID:Genetic and physical characterization of lambda transducing phages (lambda frdA) containing the fumarate reductase gene of Escherichia coli K12. 644 51

Using 200 fresh isolates of Staphylococcus epidermidis, the relationship between type of growth in soft-agar medium and respiration, dehydrogenase activity and biotype was investigated. When strains of S. epidermidis were cultured in Brain Heart Infusion medium containing 0.15% (w/v) agar, the following different growth types were observed: compact colonial morphology with growth throughout the medium (type A), or with growth only at the surface (type B); and diffuse colonial morphology with growth throughout the medium (type C), growth only at the surface (type D), or growth from the surface to the middle of the tube (type E). Five representative strains of each growth type were studied and different results for cytochrome pattern, oxygen consumption and relative activities of lactic dehydrogenase and succinic dehydrogenase were obtained with different growth types. However, there was no correlation between growth type and biotype.
J Gen Microbiol 1982 Sep
PMID:Growth of Staphylococcus epidermidis in soft agar in relation to respiration, dehydrogenase activity and biotype. 717 97

Cell envelope fractions of Moraxella nonliquefaciens were isolated by a slight modification of Osborn's method. Two main membrane fractions were characterized chemically and morphologically. The density of the fraction containing cytoplasmic membrane material was 1.17 to 1.18 g cm-3 compared with 1.24 to 1.27 g cm-3 for the outer membrane fraction. Lipopolysaccharide was detected almost exclusively in the outer membrane fraction and sodium dodecyl sulphate polyacrylamide gel electrophoresis of this fraction revealed one dominant protein band with an apparent molecular weight of 45 000. Cross-contamination of the fractions was estimated to be about 10%, as calculated on the basis of the lipopolysaccharide fatty acid 3-hydroxydodecanoic acid and on the relative activities of D-lactate dehydrogenase and succinate dehydrogenase.
J Gen Microbiol 1980 Jan
PMID:Isolation of a relatively pure outer membrane fraction of Moraxella nonliquefaciens and a comparison of its characteristics with the cytoplasmic membrane-containing material. 736 51


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