Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.3.5.1 (succinate dehydrogenase)
 8,177 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The metabolic significance of Se in plants is not well documented, though the presence of many selenoenzymes in bacteria and the essentiality of Se in higher animals is established. Since germination is an active process in plant growth and metabolism, the effect of Se was investigated in germinating Vigna radiata L, a nonaccumulating Se-deficient legume. Growth and protein were enhanced in seedlings supplemented with selenium (Se) as sodium selenite in the medium up to 1 microgram/mL. The pattern of uptake of 75Se in the differentiating tissues and the subcellular distribution were investigated. The percentage of incorporation of 75Se was greater in the mitochondria at the lowest level (0.5 micrograms/mL) of Se supplementation compared to higher levels of Se exposure. Proteins precipitated from the postmitochondrial supernatant fractions, when separated by means of polyacrylamide gel electrophoresis (PAGE), indicated a major selenoprotein in the seedlings germinated at 2.0 micrograms/mL Se. In seedlings grown with supplemented Se, enhanced respiratory control ratio and succinate dehydrogenase activity were observed in the mitochondria of tissues, indicative of a role for Se in mitochondrial membrane functions.
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PMID:Subcellular distribution of selenium during uptake and its influence on mitochondrial oxidations in germinating Vigna radiata L. 766

Requirement, uptake, and subcellular distribution of Na2(75)SeO3 in the larvae of the insect C. cephalonica was investigated. That Se is well tolerated by C. cephalonica upto an added level of 2 ppm in the diet is suggested by the observed increase in body weight, total protein, and succinate dehydrogenase levels. Significant increases in the State 3 respiration ensued with Se supplementation up to 2 ppm in the mitochondrial oxidation of D-glycerol 1-phosphate, succinate and NADH, along with concomitant unaltered State 4 respiration, leading to enhanced RCR values. Maximal uptake of 75Se was registered in the larvae maintained on basal diet when subjected to short-term exposure to 0.5 ppm 75Se level. When exposure level was further increased up to 20 ppm, the observed decrease in the uptake of 75Se suggested that Se status of larvae itself controlled the tissue uptake. Subcellular distribution pattern revealed maximal incorporation of 75Se (cpm/g tissue) in the supernatant fraction, whereas, maximal specific 75Se activity (cpm/mg protein) was associated with the mitochondrial fraction. Autoradiography of the soluble fractions indicated the presence of single selenoprotein in the larval group with short term 2 ppm 75Se exposure. Inherent Se controls both the extent and the nature of distribution of mitochondrial 75Se incorporation. Uptake of 45Ca by the insect mitochondria was enhanced by dietary Se up to 2 ppm but was unaffected by addition of in vitro 75Se in the medium. A more fundamental role for Se in the mitochondrial energy metabolism emerges from these studies.
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PMID:Metabolic relevance of selenium in the insect Corcyra cephalonica. Uptake of 75Se and subcellular distribution. 794 14