Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.3.5.1 (succinate dehydrogenase)
 8,177 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Oxidative phosphorylation and Ca2+-transport functions of liver mitochondria were normalized in rats with alloxane diabetes after peroral administration of phytoecdisteroids - ecdisterone and turkesterone (5 mg/kg) or nerobol (10 mg/kg) within 15 days. These drugs normalized the activity of NADH dehydrogenase and succinate dehydrogenase in respiratory chain of mitochondria, increased distinctly stability of the enzymes to the effect of such factors as heating, effect of phospholipase A2 or trypsin.
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PMID:[Comparative study of the effect of ecdysterone, turkesterone and nerobol on the function of rat liver mitochondria in experimental diabetes]. 377 12

The topography of phospholipids in the photosynthetic membranes of Rhodopseudomonas sphaeroides was investigated by using purified chromatophores and spheroplast-derived vesicles (SDVs). Chromatophores are closed vesicles oriented inside out with respect to the cytoplasmic membrane (cytoplasmic side out) and obtained from French-pressed cell lysates. SDVs are oriented right side out (periplasmic side out) and are obtained after osmotic lysis of lysozyme-treated cells. Phosphatidylethanolamine (PE) comprised approximately 62% and phosphatidylglycerol (PG) comprised approximately 33% of the total phospholipid of both vesicle preparations. The relatively membrane impermeable reagent trinitrobenzenesulfonate (TNBS) at 3 mM concentration and 5 degrees C modified chromatophore and SDV PE with kinetics indicating the occurrence of fast- and slow-reacting pools of PE. The fast-reacting pools comprised 33% and 55% of the total PE of chromatophores and SDVs, respectively. The slow-reacting pools comprised 61% and 32% of the total PE of chromatophores and SDVs, respectively. Phospholipase A2 treatment of chromatophores (1 unit/mg of vesicle protein) for 1 h at 37 degrees C resulted in hydrolysis of 73% and 77% of the total PG and PE, respectively. Similar enzyme treatment of SDVs resulted in 14% and 60% hydrolysis of the total PG and PE, respectively. Phospholipase A2 treatment inhibited 60% of the succinate dehydrogenase activity of chromatophores but only 8% of the activity of SDVs, indicating the membrane impermeability of phospholipase A2. Incubation of chromatophores for 10 min with 3 mM TNBS at 5 degrees C and then treatment with phospholipase A2 for 10 min and 1 h resulted in the hydrolysis of 10% and 61%, respectively, of unmodified PE. The results indicate asymmetric distributions of PE polar head groups (32-33% cytoplasmic side, 55-61% periplasmic side) and PG (73% cytoplasmic side, 14% periplasmic side) across the membrane. Also, a rapid and unidirectional transbilayer movement of PE polar head groups from the periplasmic to cytoplasmic surfaces of the membrane appears to occur during phospholipase A2 hydrolysis on the chromatophore surfaces.
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PMID:Phospholipid topography of the photosynthetic membrane of Rhodopseudomonas sphaeroides. 697 21

The development of a mitochondrial membrane permeability triggered by the Ca(2+)-stimulation of PLA2 (phospholipase A2; EC 3.1.1.4.) and based on swelling, polyunsaturated fatty acids release and calcium influx, induced the activation of SDH (succinate dehydrogenase; EC 1.3.9.9.) without damaging mitochondria structures. The activity of SDH increased within the length of permeabilization treatment before reaching a plateau. The study of Km and Vm showed that the affinity of SDH for succinate and the maximal velocity were increased. Based on these results, the change of SDH activity triggered under these conditions could be explained by a substrate activation of SDH taking account that the succinate content was significantly enhanced.
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PMID:Study of the succinate dehydrogenase activation in permeabilized mitochondria through the Ca(2+)-stimulated phospholipase A2. 783 34

Cytosolic Ca2+ overload may play a key role in the process of lead-induced retinal injury and degeneration. We report that retinal calcium content was elevated following developmental and in vitro lead exposure. To determine the concentration-dependent effects of Ca2+ (5-1000 nM) on retinal mitochondrial bioenergetics an isolation procedure was developed. Isolated mitochondria were efficiently coupled; had good respiratory control ratios with the NAD-linked substrates, glutamate or pyruvate plus malate (G/M or P/M), and the FAD-linked substrate, succinate plus rotenone (S/R); and possessed a Na+/Ca2+ exchanger. The major finding was that at equimolar [Ca2+] > or = 35 nM, mitochondria were more sensitive to and exhibited a greater degree of inhibition of coupled and uncoupled respiration with NAD-linked substrates compared to S/R. At all [Ca2+], decreases in State 3 and uncoupled respiration were similar, thereby eliminating the ATP synthase and ADP/ATP translocase as sites of inhibition and suggesting that opening the mitochondrial permeability transition pore (MTP) did not contribute to the inhibition. The effects of toxicological [Ca2+] were: (1) blocked by ruthenium red, (2) blocked by dibucaine only in the presence of NAD-linked substrates, and (3) partially reversed by NAD+ with G/M after opening the MTP. Results with G/M suggest that Ca2+ acts on the inner membrane phospholipase A2 to decrease NADH CoQ reductase activity and/or produce a NAD+ leak, whereas with S/R, Ca2+ may inhibit succinate dehydrogenase. In conclusion, Ca2+ inhibits retinal mitochondrial ATP production, which may contribute to the retinal cell injury and death observed in developmentally lead-exposed rats.
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PMID:Substrate-dependent effects of calcium on rat retinal mitochondrial respiration: physiological and toxicological studies. 817 38

Dependence of oxygen consumption by wheat root cells on the activity of phospholipase A2 (PLA2) was studied. The treatment of excised roots with 4-bromophenacile bromide (BPB), a specific inhibitor of PLA2, caused a decrease in the content of free fatty acids (FFA) and in oxygen consumption of root cells. The latter was prevented by exogenous application of a mixture of FFA. A similar inhibitory effect was caused by BPB after the activation of root respiration by 2,4-dinitrophenol (DNP). These data suggest that FFA may be involved in the regulation of respiration through the formation of succinate. This is supported by the fact of reduction of DNP-induced stimulation of oxygen consumption by malonate, known to be an inhibitor of succinate dehydrogenase, and by stimulation of respiration by exogenous application of succinate.
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PMID:[Respiration of wheat roots during inhibition of phospholipase A2 by 4-bromphenacylbromide]. 1155 49