Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.3.5.1 (succinate dehydrogenase)
 8,177 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The purpose of this work was in investigate the capability of cell extracts of Escherichia coli and E. coli treated with colicin K to catalyze the following energy-dependent reverse transhydrogenase reaction: NADP + NADH + ATP in equilibrium NADPH + NAD +ADP + Pi. Under anaerobic conditions this reaction requires the presence of a specific portion of the electron transport chain, a functional energy coupling system, including an adenosine triphosphatase, enzyme, and ATP as energy source. The ATP-linked reaction was partially inhibited in French press extracts of E. coli K-12 C600 cells that had been pretreated with colicin K but not in extracts from similarly treated cells of a colicin-tolerant mutant. Ultracentrifugation of extracts yielded particulate fractions competent in catalyzing the reaction; this reaction is substantially inhibited in fractions from colicin-treated cells. The extent of inhibition increased with increasing concentration of colicin. Supernatants also supported ATP-linked formation of NADPH, but this reaction was insensitive to the colicin effect. A comparison between the requirement of the reaction in supernatant and particulate fractions suggests that the reaction in the supernatant is different from the one inhibited by colicin. The ATP-hydrolyzing ability of particulate fractions from the control or treated bacteria was identical. Likewise, the electron transport chain was not affected by colicin treatment, as evidenced from lack of effect on NADH oxidase, succinic dehydrogenase, and NADPH-NAD transhydrogenase. It is concluded that colicin K interferes with the coupling of ATP the utilization of the intermediate for the ATP-linked transdehydrogenase reaction.
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PMID:Effect of colicin K on a membrane-associated, energy-linked function. 0 29

Immunization of rabbits with botulinus anatoxin containing a number of proteins of bacterial origin causes a statistically significant increase in the activity of succinate dehydrogenase, NAD diaphorase and NADP diaphorase as early as after 24 hours. After 5-7 days, the activity of all mitochondrial enzymes drops below the control level and returns to normal by the 14th day. The activity of glucose 6-phosphatase decreases significantly already 24 hours after immunization and returns to normal by the end of the 7th day. The mechanism of excretion of foreign protein in the kidneys of immunized animals is discussed.
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PMID:Histochemical investigation of some mitochondrial and microsomal enzymes in the kidneys of rabbits immunized with type B botulinus anatoxin. 3 81

The initial part of the axon including the axon hellock, the initial unmyelimated segment and the beginning of myelinated axon was studied electron microscopically during regeneration, 1--30 days following a crush lesion of the rat hypoglossal nerve. Large mitochondria reaching 1.1 mum in diameter, with abundant cristae and dense granules in the matrix were observed between days 3--21. They formed clusters in the initial myelinated segment of the axon. End-to-end contacts and ribosomes around them were very often visible. The large mitochondria exhibited strong succinate dehydrogenase and NAD - H2 diaphorase activities. The relationship between the appearance of large and active mitochondria in the initial part of the axon and the elevated axonal transport during regeneration of the peripheral nerve is also discussed.
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PMID:Changes in the mitochondria in the initial part of the axon during regeneration. 6 4

The distribution of succinic dehydrogenase, HApi-diaphorase, glucose-6-phosphatdehydrogenase, alkaline and acid phosphatases and glucose-6-phosphatase was studied by means of the incubation of whole cestodes. Succinic dehydrogenase, NAD-diaphorase and glucose-6-phosphatdehydrogenase are connected in general with the fixating apparatus of the scolex and genital organs; phosphatases -- with the integument tissues, excretory system and calcareous corpuscles. The results obtained are in complete agreement with the available data on the distribution of the enzymes studied. The incubation method of whole cestodes can be useful for field works.
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PMID:[Distribution of certain enzymes in totally stained Cestode preparations]. 6 56

The research was carried out on albino rats. The animals in the experimental group were given Andiamina (Hexobendine) in a dose of 40 mg/kg for a period of 7 days in the group I and 21 days in the group II. The results have pointed out that changes in the activity of the studied enzymes occurred especially after 21 days of Hexobendine administration. First of all, it caused a decrease in lactic and glucose-6-phosphate dehydrogenase activities and to lesser degree, it influenced the activities of iso-citric dehydrogenase and NAD and NADP tetrazole reductases. At the same time, reaction to succinic dehydrogenase indicated an increase in the enzymatic activity.
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PMID:The influence of Andiamina (hexobendine) on the histochemical reaction in the aorta wall of experimental animals. 11 27

A reduction in the content of neutral mucopolysaccharides in mucous cells of the neck, a slight decrease in the activity of succinate dehydrogenase and NAD-diaphorase in parietal cells, a decrease in the DNA synthesis rate, and an increase in the area of mitochondria and cristae were detected in the gastric mucosa of rats which were in a long-term space flight. In the small intestine, an increase in the activity of glucose-6-phosphate dehydrogenase and leucine aminopeptidase were found. Morphological changes in the liver consisted in infiltrative adiposity. A similar morphological picture was demonstrated in a synchronous experiment on the earth. These changes, however, were nonspecific and reversible (25 days after rehabilitation the picture did not differ from the animal house control).
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PMID:[Morphological changes in the digestive organs during prolonged space flight on the Kosmos-782 biosatellite]. 15

In cells of human embryo skin--muscle tissue transformed by the Rouse sarcoma virus (23rd cell line) and polyoma virus (P-2 cell line), the mitotic activity was 48 0/00 for 23rd line, 51 0/00 for P-2 line as against 28 0/00 in the control cells. The transformed cells possessed greater amounts of RNA and DNA and protein--bound SH-groups, different forms of glycogen deposits, as well as higher acid phosphatase enzyme activities; there was practically no difference in acid mucopolysaccharide content or NAD-H2-diaphorase and succinate dehydrogenase activities.
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PMID:[Morphological and cytochemical characteristics of human cells transformed and made malignant by Rous and polyoma viruses]. 16 14

The distribution of succinic dehydrogenase, (see article), glucose-6-phosphat-dehydrogenase, alkaline and acid phosphatases and glucose-6-phosphatase was studied by means of the incubation of whole cestodes. Succinic dehydrogenase, NAD-diaphorase and glucose-6-phosphatdehydrogenase are connected in general with the fixating apparatus of the scolex and genital organs; phosphatases -- with the integument tissues, excretory system and calcareous corpuscles. The results obtained are in complete agreement with the available data on the distribution of the enzymes studied. The incubation method of whole cestodes can be useful for field works.
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PMID:[Distribution of some enzymes in totally stained preparations of cestodes]. 17 33

The article deals with oxidation of different substrates, intensity of glycolytic and glycogenolytic processes in mitochondria and homogenates of dog liver with its 2-hour exclusion from circulation under conditions of endotracheal ether-oxygen narcosis. It was established that already 30-60-minute ischemia causes a decrease in intensity of succinate, alpha-ketoglutarate oxidation and acceptor respiration, inhibiton in the activity of the citrate cycle enzymes; succinate dehydrogenase, alpha-ketoglutarate dehydrogenase, isocytrate dehydrogenase. The activity of NAD-dependent malate dehydrogenasedehydrogenase and Mg2+-ATPase as well as intensity of NADN oxidation in mitochondria increase. After 2-hour ischemia the activity of Mg2+-ATPase, cytochrome oxidase and peroxidase lowers. A sharply developed glycogenolysis is accompanied by inhibition of phosphorylase activity and a two-fold stimulation of the glycolytic reactions. Peculiarities in regulation of enzymatic reactions under conditions of ischemia and their role in origin of metabolism disturbances in the liver are under discussion.
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PMID:[Carbohydrate metabolism in the liver in acute ischemia]. 17 60

The effect on rat myocardium of autolysis at 19 degrees C, for up to 20 days, was studied by electron microscopy and enzyme histochemistry. The enzymes studied included monoamine oxidase (MAO), sytochrome oxidase (CytO), non-specific esterase ((Ns.E.), phosphorylase (P-ase), succinate dehydrogenase (SDH) and various NAD- and NADP-linked dehydrogenases. The myocardium lost its histochemical P-ase activity within a few hours of autolysis, whereas the activity of all other enzymes remained quite normal for at least about 4 days, except that of MAO and SDH, which were normal for about 8 and 12 days, respectively. The myocardial cells lost activity of various enzymes in a patchy manner during prolonged autolysis and practically all histochemical enzyme activity disappeared within 20 days. The early period of autolysis was accompanied by rapid ultrastructural changes of myocardial cells. During prolonged autolysis the gross architecture of the myocardium was lost gradually by the 12th to 20th days. Mitochondria were the organelles most resistant to the effects of autolysis, and numerous mitochondria with morphologically solid inner and outer membranes were seen among the totally disintegrated myocardium 20 days after death. The loss of P-ase activity coincided with the loss of glycogen. The loss of MAO, SDH and CytO activities was not closely related to the morphological preservation of mitochondria, but, in accordance with other enzymes, was more closely related to the disintegration of the over-all myocardial structure. The present results showed that the architecture of the myocardium, and especially that of the mitochondria, was surprisingly resistant to the effects of autolysis at room temperature. Also several enzymes of the myocardium other than those examined so far maintained quite stable histochemically demonstrable activity during prolonged autolysis. These observations give support to the possibility of making the diagnosis of myocardial infarction at postmortem more accurate than with the present morphological and histochemical routine methods.
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PMID:Electron microscopical and enzyme histochemical changes in the rat myocardium during prolonged autolysis. 17 22


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