Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.3.5.1 (succinate dehydrogenase)
 8,177 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The nearest neighbourhood of pigment-protein complexes within Photosystem II (PSII) membrane fragments has been studied by means of chemical cross-linking with o-phthalaldehyde (OPA) in conjunction with protein-chemical techniques. By means of OPA-induced cross-linking a major conjugate of about 60 kDa has been identified. This conjugate was shown to consist of two pigment-protein complexes of light-harvesting complex II (LHC II), Lhc b1 (CP27) and Lhc b4 (CP29) by means of SDS/PAGE in combination with an immunological analysis using mAbs directed against Lhc b4 and by matrix-assisted-laser-desorption-ionization mass spectrometry (MALDI-MS) and sequence analysis of peptides derived from a proteolytic digest of the conjugate. Domains of Lhc bl and Lhc b4 have been localized to a distance of not more than 5 A within LHC II. Our results are discussed in the light of recent models on the topography of the two subunits within the antenna system of Photosystem II.
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PMID:Close vicinity of Lhc b1 and Lhc b4 in Photosystem II--membrane fragments as verified by chemical cross-linking. 983 47

Various extracts of brain were prepared with dilute and concentrated acid, acetone and n-butanol. When the extracts were applied to the guinea-pig isolated ileum, they produced a slow contraction which was not prevented by an antihistamine; in addition, the acid and acetone extracts inhibited the action of histamine. Histamine in extracts of brain was separated from other pharmacologically active substances by chromatography on a carboxylic-acid resin and estimated biologically. The bulk of this histamine was found in small particulate material, whereas slow-reacting substance was found in particulate material of greater density. 5-Hydroxytryptamine was in both the small and large particles. There was no relationship between the distribution of these substances and that of succinic dehydrogenase activity. The measurement of histamine in brain both by biological assay on the guinea-pig ileum and by chemical assay using a fluorimetric procedure gave mean values of 53 and 246 ng/g of wet tissue, respectively. The high values obtained by the chemical assay are attributable in part to substances other than histamine which become fluorescent after reaction with o-phthalaldehyde in this procedure.
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PMID:The subcellular distribution of histamine, slow-reacting substance and 5-hydroxytryptamine in the brain of the rat. 1401 77