Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.3.5.1 (succinate dehydrogenase)
 8,177 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The activities of L-threonine dehydrogenase (I), 2-amino-3-oxybutyrate:CoA ligase (II), malate synthetase (III), isocitrate lyase (IV), glyoxylate dehydrogenase (V), glycine decarboxylase (VI), L-serine hydroxymethyltransferase (VII), glucan synthetase (VIII), glucose 6-phosphate dehydrogenase (IX) and succinic dehydrogenase (X) were detected in cell-free extracts prepared from the mycelium of the fungus Sclerotium rolfsii type R. Transfer of S. rolfsii to a threonine-containing medium resulted in a significant increase in the intracellular concentrations of L-threonine, glycine, serine and glyoxylate, and a decrease in oxalate. Incubation with 14C-labelled L-threonine resulted in an immediate output of 14CO2, and an accumulation of labelled glycine and serine in the mycelium. L-Threonine (10(-2)M) increased branching, favoured formation of sclerotia, and induced the formation of enzymes I to VIII, but not IX and X. Sodium oxalate (1-5 X 10(-2)M) inhibited branching, sclerotium formation and the activity of enzymes III and IV. Glycine (10(-1) M) inhibited branching, sclerotium formation and activity of I and II. Ammonium chloride (10(-1) to 10(-2) M) inhibited formation of sclerotia, threonine uptake and activity of III. Acetyl-CoA inhibited V and L-cysteine inhibited I as well as sclerotium formation and branching. It is suggested that hyphal morphogenesis and formation of sclerotia in S. rolfsii require an increased supply of carbohydrate intermediates and energy and that these are mainly supplied by the glyoxylate pathway.
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PMID:Metabolism of L-threonine and its relationship to sclerotium formation in Sclerotium rolfsii. 98 16

Pseudomonas sp. C27 is a facultative autotrophic bacterium that can effectively conduct mixotrophic and heterotrophic denitrification reactions using organic matters and sulfide as electron donors. There is no experimental confirmation on proteomic levels the pure C27 strain can have the capability to simultaneous removal of sulfide, nitrate and organic carbon from waters. The proteome in total C27 cell extracts was observed by two-dimensional gel electrophoresis. The 160mg/L sulfide up-regulated or specifically expressed succinate dehydrogenase, iron-sulfur protein, oxidoreductase, serine hydroxymethyltransferase, and iron superoxide dismutase for sulfide metabolism, 2-oxoglutarate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, NAD(+)-dependent aldehyde dehydrogenase, malate dehydrogenase and succinate dehydrogenase for carbon metabolism, and nitrous-oxide reductase and respiratory nitrate reductase for nitrogen metabolism. The study confirmed that the C27 strain has an effective enzyme system to conduct denitrifying sulfide removal reactions. Also, sulfide stress can enhance energy consumption rate and rates of nitrate reduction and sulfide oxidation by C27. Conversely, sulfide stress repressed the sulfate-reducing power of C27, evidenced by down-regulation or specific un-expression of sulfate ABC transporter, periplasmic sulfate-binding protein in the (C+N+S) sample.
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PMID:Sulfur-nitrogen-carbon removal of Pseudomonas sp. C27 under sulfide stress. 2368 98