Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.3.5.1 (succinate dehydrogenase)
 8,177 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The specific activity of a peroxisomal enzyme, lactate oxidase, and of pyruvate kinase and lactate dehydrogenase, which are not peroxisomal, increased rapidly when shaken cultures of Tetrahymena were transferred to conditions of oxygen restriction and supplemented with glucose. Two other peroxisomal enzymes, catalase and TPN-linked isocitrate dehydrogenase, did not increase substantially, nor did succinate dehydrogenase. The increases were reduced if glucose was not added at the time of transfer, and were prevented by actinomycin D or cycloheximide, but not by chloramphenicol. The results suggest an involvement of peroxisomes in the metabolism of glycolytic endproducts when the availability of oxygen to the cell is limiting.
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PMID:Synthesis of glycolytic and peroxisomal enzymes in Tetrahymena following a change in culture conditions. 80 70

Growth of Mycobacterium phlei under low oxygen tension resulted in specific activities two to twenty times lower for formate dehydrogenase, malate dehydrogenase, beta-hydroxybutyrate dehydrogenase, lactate oxidase and NADH dehydrogenase than when cultures were grown under high aeration. An increase in fumarate reductase and succinate dehydrogenase occurred with M. phlei grown under low oxygen tension. Malate: vitamin K dehydrogenase and glucose-6-phosphate dehydrogenase activity were not significantly affected by the oxygen tension used to grow the bacteria, and neither culture contained a lactate dehydrogenase. With growth of M. phlei in conditions of low oxygen tension, cytochrome a was not detected, but cytochrome b was prominent in membranes and cytochrome c was present in the soluble fraction.
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PMID:Influence of oxygen tension on the respiratory activity of Mycobacterium phlei. 318 14

Membranes obtained from Escherichia coli have been solubilized with deoxycholate. The solubilized dehydrogenases and cytochromes are not sedimented at 105,000 g. These components readily penetrate the "included space" of Sepharose 4B (Pharmacia Fine Chemicals Inc., Uppsala, Sweden) and polyacrylamide gels and have been fractionated on the basis of molecular size. Solubilization destroys nicotinamide adenine dinucleotide, reduced form (NADH) oxidase and D-lactate oxidase activities, but leaves an appreciable part of the original succinoxidase activity intact. Evidence for a succinate dehydrogenase-cytochrome b(1) complex is given. Menadione added to the solubilized preparation does not elicit NADH oxidase activity nor stimulate the existing succinoxidase activity, but does provoke an active D-lactate oxidase activity. This D-lactate oxidase activity, however, does not use cytochromes and is not sensitive to cyanide.
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PMID:Respiration and protein synthesis in Escherichia coli membrane-envelope fragments. VI. Solubilization and characterization of the electron transport chain. 440 70

The effects of membrane composition on the sensitivity of membrane functions to ethanol in Escherichia coli have been investigated. The addition of ethanol (0.67M) in vitro did not cause appreciable inhibition of NADH oxidase, D-lactate oxidase or ATPase but caused an 11% to 30% inhibition of succinic dehydrogenase, glutamate uptake, proline uptake and 1ac permease. Although the sensitivities of some of these membrane functions to ethanol varied with membrane composition, none correlated with the changes in sensitivity to killing by ethanol. In contrast, leucine transport was resistant to ethanol (0.67M) in control cells and in cells enriched in vaccenic acid, but was inhibited by 25% in cells grown in palmitic acid. The release of nucleotides was examined as a comparative measure of cellular permeability. Ethanol increased nucleotide leakage. Leakage was reduced in cells grown in vaccenic acid and enhanced in cells enriched in palmitic acid. The addition of MgSO4 (10mM) reduced nucleotide leakage and enhanced survival. Based upon these results, metabolite leakage was proposed as the primary event associated with bacterial inactivation in buffered solutions of ethanol. The increase in acyl chain length is proposed as the beneficial aspect of vaccenic acid incorporation rather than the increase in membrane unsaturation.
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PMID:Effects of fatty acid composition on the sensitivity of membrane functions to ethanol in Escherichia coli. 675 94