Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.3.5.1 (succinate dehydrogenase)
 8,177 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Binding studies with (-)-[125I]cyanopindolol (ICYP) were conducted to characterize beta-adrenoceptors in plantaris and soleus muscles of rats (male, 250-300 g). The distribution of beta 1- and beta 2-adrenoceptors in different muscle fiber types, identified in serial sections by succinic dehydrogenase (SDH) staining, was studied by autoradiography. The densities of binding sites (Bmax, fmol/mg protein) were 5.4 +/- 0.9 (mean +/- SEM) in plantaris and 11.5 +/- 2.0 in soleus muscle. In plantaris muscle, monophasic competition curves were observed when binding experiments were performed using CGP20712A (50 pM to 0.5 mM), a beta 1-adrenoceptor selective antagonist, or ICI 118,551 (50 pM to 20 microM), a beta 2-adrenoceptor selective antagonist, to compete for ICYP binding. Analysis with LIGAND revealed a single binding site with a KD value of 2.41 +/- 0.56 nM (mean +/- SEM) for ICI 118,551 and 8.93 +/- 3.00 microM for CGP 20712A, indicating the presence of a homogeneous population of beta 2-adrenoceptors. In soleus muscle, competition curves were biphasic with 16-21% beta 1-adrenoceptors. Autoradiographic studies supported the findings from binding studies with membrane homogenates. The ICYP binding pattern was associated closely with the muscle fiber types identified by SDH staining. Propranolol-resistant binding sites were observed, and these sites were associated with muscle fibers positive to SDH staining.
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PMID:Characterization of beta 1- and beta 2-adrenoceptors in rat skeletal muscles. 168 10

The possible effect of practolol (ICI 50,172) on mitochondrial metabolism was studied. The drug inhibited the oxidation of glutamate, alpha-ketoglutarate and succinate by heart mitochondria. The polarographic determinations showed that practolol is an inhibitor of the oxidative phosphorylation. The activity of NADH-oxidase, NADH-ferricyanide reductase, NADH-cytochrome c redutase was inhibit by the drug; no effect was observed on the succinate dehydrogenase. The electron microscopy of isolated mitochondria treated with practolol showed that the drug promote conformational changes of the mitochondrial membrane, probably concerning with the detergent characteristics of the drug.
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PMID:Studies of practolol on mitochondrial metabolism. 740 72