Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.3.5.1 (succinate dehydrogenase)
 8,177 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An optimal and practical method for high resolution separation and visualization of soluble and membrane-binding isoenzymes is described. By use of this method, non-specific esterase, lactate dehydrogenase and acid phosphatase from different tissues of mice can be separated into more than 50, 30 and 20 components, respectively. Cholinesterase, glucose-6-phosphate dehydrogenase, acid phosphatase, succinate dehydrogenase, lactate dehydrogenase and non-specific esterase isolated from the lumbar spinal cord of chick embryos at different ages can be separated into about 15, 12, 9, 6, 14 and 20 components, respectively. In addition, an attempt has been made to evaluate quantitatively by densitometry differences in enzyme activity among different regions of the same tissue (e.g. spinal cord). The findings revealed that there are differences in isoenzyme components between the dorsal and ventral regions of the spinal cord. Collectively, the combination of direct tissue isoelectric focusing and fast enzyme visualization reveals a greater number of isoenzyme components than can be demonstrated by other means. This method is an extremely useful procedure for understanding and analyzing the nature and topographic localization of isoenzyme components.
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PMID:Histo-electrophoretic analysis of the microheterogeneity and region-specificity of isozymes. 175 87

The histochemical pattern of muscle fiber types of the longissimus dorsi and biceps femoris muscles was investigated in normal and splaylegged piglets at birth and seven days later. Only slight differences between the muscle fibers at birth were found using histochemical reactions for alkaline adenosine triphosphatase (ATPase), succinate dehydrogenase (SDH), phosphorylase (PH) activities, and for the periodic acid-Schiff (PAS) reaction. With the method for acid-preincubated ATPase activity, high activity was observed in Type I muscle fibers and low activity in Type II muscle fibers in animals of both groups investigated. However, a higher number of Type I fibers was found in muscles of normal piglets, suggesting a faster and more advanced process of transformation of Type II into Type I muscle fibers in unaffected animals. Thus the histochemical conversion appears to be retarded in muscles of splaylegged animals, which have a histochemical pattern similar to that of normal prenatal animals. Cholinesterase activity in motor endplates was well developed; its staining revealed smaller sized and irregularly arranged endplates in muscles of affected piglets. Fiber type differentiation in muscles of animals which recovered from splayleg becomes fully developed and comparable to normal piglets seven days after birth. The number of fibers which became converted from Type II to Type I was increased; the fiber types were differentiated with regard to the PAS reaction and to their ATPase, SDH and PH activities. Morphological features of motor endplates in muscles of normal and surviving splaylegged piglets are similar. Histochemical investigation of the fiber type differentiation thus suggests that full recovery occurs within the first week of postnatal life in muscles affected by pathological changes accompanying splayleg.
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PMID:Histochemical patterns in normal and splaylegged piglet muscle fibers. 644 32

The aim of the present investigation was to detect the regularities of postnatal development of "motor end-plate-muscle fiber (MF)-vascular network" system in different calf muscles of intact albino rats. Gastrocnemius, plantaris and soleus muscles were studied in 72 albino rats aged from 14 to 180 days. Identification of MF type was performed on the basis of succinate dehydrogenase and NADH-diaphorase activity. Cholinesterase activity of the neuro-muscular synapse (NMS) and alkaline phosphatase activity in the vascular endothelium were demonstrated using a combined histochemical method. The diameter of vascular network and the number of enzyme-active zones (EAZ) per one MF were the earliest parameters to be stabilized (before day 30). Histochemical profile of skeletal muscle was stabilized by the end of day 60. Dynamics of MF diameter and EAZ in NMS, vessel diameter and numbers per one MF is characterized by the periods of active changes (days 14-30), decrease (days 30-60) and stabilization (after day 60) of variance rate. The association between the level of oxidative metabolism and MF diameter was demonstrated.
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PMID:[Differentiation of calf skeletal muscles in the postnatal period of ontogenesis]. 2096 Jul 12