Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.3.5.1 (succinate dehydrogenase)
 8,177 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In the periosteum of newborn, 8-, 30- and 60-day-old albino rats, beta-glucuronidase, beta-galactosidase and succinate dehydrogenase were found. The enzymes of the investigated age groups show remarkable differences in reaction. beta-Glucuronidase reacts distinctly in the cells of the cambium layer of 8- and 30-day-old animals. beta-Galactosidase reacts very differently in the cell layers of the periosteum of albino rats in all age groups. The reaction of succinate dehydrogenase we found above all in the cambium cells, the preosteoblasts and the osteoblasts.
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PMID:[Studies of enzyme distribution in carbohydrate metabolism in humeral periosteum in rats from different age groups]. 89 10

The influence of irradiation was studied histochemically in healing mandibular periosteum and bone. After a cut line had been made on both sides of the mandible the rats were exposed to roentgen ray irradiation. The single doses were 15, 20, 30, 35 or 40 Gy. The animals were killed 1, 2, 4, 8, 10, 12, 16 and 24 hours after irradiation, for histochemical analysis. All enzymes, acid phosphatase, cytochrome oxidase, lactate, isocitrate, glucose-6-phosphatase and succinate dehydrogenase, showed a greater increase in enzyme staining in the irradiated cut lines than in the non-irradiated control lines. The intensity of the staining increased with time and dose over 24 hours. The observation time included an inflammatory phase with vascular, enzymatic and cellular responses to periosteal and bone injury. The increase in staining was dependent on the time after surgical trauma and radiation dose. The increase in enzyme staining probably represents the initial cell damage after irradiation.
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PMID:Effect of irradiation on early enzymatic changes in healing mandibular periosteum and bone. A histochemical study on rats. 302 Aug 89

The present study was conducted on bone tissue responses to irradiation towards a treatment model of mandibular irradiation injury by comparing the results of experimental observations of irradiation effects on rabbit hind legs and rat mandibular bones (paper I, II and III) with clinical observations of irradiation effects on the human mandible (paper IV, V and VI). The main results of the study were as follows: Bone marrow haemorrhage, eosinophilia and incipient edema were encountered in the rabbit leg one day after a single irradiation dose. Edema and fibrosis were the salient features after five weeks, while both regenerative and fibrotic changes predominated eleven weeks after irradiation. The changes were the more extensive the greater the irradiation dose was. Empty lacunae as a sign of cell damage in cortical bone already appeared on the first day after irradiation; this effect reached its maximum when the dose was 20 Gy or more. Bone marrow and subcutaneous tissue pO2 and pCO2 were measured by means of implanted Silastic tonometers in irradiated and nonirradiated rabbit hind legs. Single dose irradiation was followed by a rapid, dose dependent decrease of marrow pO2. The corresponding effect on pCO2 was weaker and appeared later. The response to hyperoxia in the bone marrow became weaker when the irradiation dose increased. Less significant was the response of CO2 tension to hyperoxia. O2 and CO2 tensions were recovered after single dose irradiation both in subcutaneous tissue and in bone marrow, but the reduction was less in bone marrow. During the twelve weeks observation period clearly better recovery in tissue gas tensions was observed in subcutaneous tissue than in bone marrow. Nonirradiated periosteal grafts on irradiated bone cavities in the rabbit tibia induced more rapid and intense mature bone formation than irradiated periosteal grafts. The irradiated periosteum, even after a single dose of 20 Gy, had some osteogenetic capacity. The alkaline phosphatase content was lowered eight weeks after surgery in irradiated legs but clearly exceeded control values twelve weeks after surgery indicating new bone formation. Lysosomal enzyme DAP II contents were increased in all irradiated specimens as a sign of disturbed bone formation. The tissue concentrations of acid phosphatase, cytochrome oxidase, lactate dehydrogenase, isocitrate dehydrogenase, glucose-6-phosphate dehydrogenase and succinate dehydrogenase in the immediate postirradiation period showed a greater increase in activity in the cut lines of the irradiated rat mandibles than in those of the nonirradiated mandibles.(ABSTRACT TRUNCATED AT 400 WORDS)
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PMID:Bone tissue response to irradiation and treatment model of mandibular irradiation injury. An experimental and clinical study. 309 Aug 54

In this work the biocompatibility of porous bioceramic implanted to the rabbit femoral bone was studied. The animals were killed 3, 6, 9, 14, 18 and 30 days after implantation and the callus with surrounding periosteum from the site of implant was taken for the studies. Morphological investigations of the callus were carried out up to the 30th day of healing of the bone tissue. Moreover, acid mucopolysaccharides level and activity of enzymes (acid and alkaline phosphatase, aminopeptidase, non-specific alpha-esterase, adenosine triphosphatase and succinate dehydrogenase) were studied up to the 18 day of the callus development. The results show that after bioceramic implantation, morphology of particular stages of the callus development, behaviour of acid mucopolysaccharides as well as localization and activity of enzymes are the same as in the normal healing process of the injured bone tissue. After 30 days total union of the mature bone tissue with bioceramic was established. We conclude that porous bioceramic satisfies the requirements for biomedical materials and may be safely used in the treatment of certain bone system diseases in humans.
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PMID:Application of porous bioceramic in experimental therapy of bone injuries. III. Dynamics of the callus development at the site of porous bioceramic implantation. Morphological, histochemical and histoenzymological studies. 323 60

Osteoclast formation in the microphthalmic osteopetrotic (mi) mouse was studied from very early embryonic to newborn stages. Embryonic and fetal milmi osteoclasts, generated during the period before bone marrow is formed in the long bones, were predominantly mononuclear and lacked ruffled borders. These cells did, however, show many osteoclastic morphologic and functional properties, such as an abundance of mitochondria, positive succinic dehydrogenase and acid phosphatase reactions, and close contact with and resorption of the calcified cartilage matrix (though diminished). These osteoclastic mononuclear cells appeared in vivo as well as in organ cultures of fetal metatarsal bones with their intact periostea. They also were observed in cocultures of periosteum-free fetal metatarsal bones, with several extraneous sources of osteoclast precursors: yolk sacs and abdominal regions of 9- and 11-day-old embryos, fetal livers, and precultured mononuclear phagocytes isolated from the fetal liver. In contrast, +/+ osteoclasts were always multinuclear, functioned normally in resorbing the calcified cartilage matrix, and had ruffled borders in vivo as well as when derived from the above-mentioned sources. Fetal liver-derived milmi macrophages also failed to form multinuclear foreign body giant cells as opposed to +/+ macrophages in granulomas on implanted pieces of Melinex. The fusion failure of cells derived from embryonic and fetal extramedullary milmi monocyte/macrophage sources contrasted with the occurrence of multinuclear osteoclasts and foreign body giant cells derived from precursors from the bone marrow in young milmi mice. We conclude that the fusion defect of milmi osteoclast precursor cells is already present in their ancestry in blood cell-forming organs of very young embryos and that these cells differentiate into mononuclear osteoclasts that function inefficiently in prenatal bone. We presume that in fully developed bone marrow, local factors are favorable for abolishing the fusion defect.
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PMID:Fusion disability of embryonic osteoclast precursor cells and macrophages in the microphthalmic osteopetrotic mouse. 399 57