Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.2.1.13 (
glyceraldehyde-3-phosphate dehydrogenase
)
6,511
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Mycoplasma suis (M. suis) is an uncultivable haemotrophic mycoplasma that parasitizes the red blood cells of a wide range of domestic and wild animals. Adhesion of M. suis to host erythrocytes is crucial for its unique RBC-dependent lifecycle. MSG1 protein (now named as
GAPDH
) with homology to
glyceraldehyde-3-phosphate dehydrogenase
(
GAPDH
) was the first identified adhesion protein of M. suis. In this study, we found that
O-sialoglycoprotein endopeptidase
(
OSGEP
) is another M. suis protein capable of binding porcine erythrocytes. Recombinant
OSGEP
expressed in E. coli demonstrated surface localization similar to
GAPDH
. Purified rOSGEP bound to erythrocyte membrane preparations in a dose-dependent manner and this adhesion could be specifically inhibited by anti-rOSGEP antibodies. E. coli transformants expressing
OSGEP
on their surface were able to adhere to porcine erythrocytes. Furthermore, using far-western and pull-down assays, we determined the host membrane proteins that interacted with
OSGEP
and
GAPDH
were Band3 and glycophorin A (GPA). In conclusion, our studies indicated that
OSGEP
and
GAPDH
could interact with both Band3 and GPA to mediate adhesion of M. suis to porcine erythrocytes.
...
PMID:Identification of erythrocyte membrane proteins interacting with Mycoplasma suis GAPDH and OSGEP. 2989 Mar 85
