Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.17.3.2 (xanthine oxidase)
 8,383 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Phenylpropanoid glycoside acteoside was extracted from the traditional Chinese medicine Scrophularia ningpoenis Hemsl. In the present study, we investigated the effects of acteoside administration on serum uric acid levels in mice rendered hyperuricemic with the uricase inhibitor potassium oxonate. When administered orally for 3 days at doses of 50, 100 and 150 mg/kg, acteoside reduced serum uric acid levels by 15.2, 23.8 and 33.1%, respectively, relative to vehicle-treated hyperuricemic mice. Importantly, in non-hyperuricemic mice, the serum uric acid levels were not affected by acetoside treatment. Acteoside also inhibited mouse liver xanthine dehydrogenase XDH and xanthine oxidase XO activity at all three doses. These results suggest that the hypouricemic action of acteoside may be attributable to its inhibition of XDH/XO activity.
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PMID:Hypouricemic effects of phenylpropanoid glycosides acteoside of Scrophularia ningpoensis on serum uric acid levels in potassium oxonate-pretreated Mice. 1830 58

A multi-hyphenated analytical method that was successfully established in previous research was applied to quality evaluation of traditional Chinese medicine (TCM) to verify its feasibility in complex systems. Scutellariae Radix (SR), which significantly protects against oxidative damage from ischemia and reperfusion, was selected as the TCM for this study. A dual-activity detection system based on xanthine oxidase (XOD) inhibition and superoxide anion (O2-) scavenging activity was used to generate a multi-dimensional-multi-informational (MD-MI) integrated fingerprint of SR. Combined with HPLC-ESI-Q-TOF-MS analysis, 17 active compounds in SR were tentatively identified by comparison with reference substances or literature data. The quality of SR from different habitats was comprehensively and systematically evaluated in respect of chemical composition, XOD inhibition and O2- scavenging activity. It was confirmed that SR contains many antioxidants and XOD inhibitory substances with diverse functions. Among them, baicalin, norwogonin-7-O-glucuronide and baicalein are the main contributors to direct antioxidant activity. Acteoside, 5,7,2',5'-tetrahydroxy-8,6'-dimethoxy flavone, baicalin and baicalein are the main XOD inhibitory components of SR. Comprehensive analysis found that the antioxidant activity of SR from Gansu Province was superior to that from other provinces in terms of both XOD inhibition and O2- scavenging activity. It has been demonstrated that the method is capable of analyzing complex TCM matrices, and can provide a useful reference for establishing quality control of TCM from the perspective of MD-MI.
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PMID:Application of multi-dimensional and multi-informational (MD-MI) integrated xanthine oxidase and superoxide anion fingerprint in quality evaluation of Scutellariae Radix. 3290 58