Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.16.3.1 (
ceruloplasmin
)
5,074
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Type 2 diabetes mellitus (T2DM) is associated with oxidative stress and perturbed iron metabolism. Serotransferrin (
Trf
) and
ceruloplasmin
(Cp) are two key proteins involved in iron metabolism and anti-oxidant defense. Non-enzymatic glycation and oxidative modification of plasma proteins are known to occur under hyperglycemia and oxidative stress. In this study, shotgun proteomics and
2
H
2
O-based metabolic labeling were used to characterize post-translational modifications and assess the kinetics of
Trf
and Cp in T2DM patients and matched controls in vivo. Six early lysine (Amadori) and one advanced arginine glycation were detected in
Trf
. No glycation, but five asparagine deamidations, were found in Cp. T2DM patients had increased fractional catabolic rates of both
Trf
and Cp that correlated with HbA
1c
(p < 0.05). The glycated
Trf
population was subject to an even faster degradation compared to the total
Trf
pool, suggesting that hyperglycemia contributed to an increased
Trf
degradation in T2DM patients. Enhanced production of
Trf
and Cp kept their levels stable. The changes in
Trf
and Cp turnover were associated with increased systemic oxidative stress without any alteration in iron status in T2DM. These findings can help better understand the potential role of altered
Trf
and Cp metabolism in the pathogenesis of T2DM and other diseases.
...
PMID:Increased serotransferrin and ceruloplasmin turnover in diet-controlled patients with type 2 diabetes. 2907 28
