Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.16.3.1 (ceruloplasmin)
 5,074 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Exposure to molybdenum in dust was measured in a molybdenite roasting plant. This exposure was accompanied by large elevations of serum ceruloplasmin and smaller increases in mean serum uric acid levels in the workers. Absorption of molybdenum from the dust was demonstrated by increases in plasma and urinary molybdenum levels. It remains necessary to demonstrate whether such exposure results in long-term health effects.
 ...
PMID:Biochemical abnormalities in workers exposed to molybdenum dust. 49 25

1. A 30-week study has been made in growing ewe lambs of the effects of dietary supplementation with molybdenum, or Mo plus inorganic sulphate on the distribution of copper in their blood plasma and kidneys. 2. The addition of 25 mg Mo and 5 g SO42-/kg diet increased Cu concentrations in plasma and kidney and decreased those in liver. Plasma caeruloplasmin activities (ferroxidase; EC 1.16.3.1) were unaffected. 3. Subcellular fractionation of the kidney cortex and gel filtration of the plasma and kidney cortex cytosol showed that the increased Cu content of these tissues was associated with abnormalities in the distribution of Cu. It appeared that both Cu and Mo were associated with the same proteins. 4. Dietary supplementation with Mo alone (25 mg/kg) had no effect on plasma or kidney Cu concentrations, suggesting that S metabolism is involved in the formation of the abnormal Cu-binding proteins in plasma and kidney. 5. The results are discussed in relation to the induction of Mo-induced Cu-deficiency states and the possible involvement of thiomolybdates.
 ...
PMID:Effects of dietary molybdenum and sulphur on the distribution of copper in plasma and kidneys of sheep. 62 21

On separate occasions young cattle were injected intrarumenally with 99Mo or 64Cu or intravenously with 64Cu. The metabolism of the isotopes were compared to evaluate metabolic changes associated with molybdenum-induced copper deficiency. Molybdenum-99 metabolism was the same in both controls and experimental subjects. Marked differences in plasma kinetics were observed following intrarumenal 64Cu injection, with experimental animals displaying earlier plasma appearance and maintaining higher plasma levels than did controls. Similarly, higher plasma levels, more rapid plasma reappearance and greater fecal excretion were observed following intravenous injection of 64Cu in the experimental animals than in controls. A mechanism involving abnormal ceruloplasmin synthesis associated with the molybdenum-copper interaction is proposed.
 ...
PMID:Effects of dietary molybdenum on the metabolism of copper and molybdenum in young cattle. 95 43

An experiment was designed to investigate the practical possibility of incorporating small quantities of molybdenum salts into the high copper diets of intensively fattening lambs to prevent or reduce the gradual accumulation of copper from feed. At slaughter (14 weeks of age) lambs which had received Mo supplement (7.7 ppm Mo) showed liver copper levels which were 40.1 per cent lower than those in the control group which, in turn, presented a mean liver copper concentration near the upper limit of the normal range for adult sheep. Changes in blood ceruloplasmin and SGOT levels and in the comparative rate of accumulation of liver copper indicated that the addition of ammonium molybdate to the concentrate diet might be a useful method of reducing the risk of nutritional copper poisoning in housed sheep.
 ...
PMID:The use of molybdenum for the prevention of nutritional copper poisoning in housed sheep. 98 81

In sheep from biogeochemical provinces enriched by molybdenum and copper and in a model form of molybdenum toxicosis in animals, the important role of enzymic and neurohumoral systems in the development of adaptation to excessive uptake of molybdenum and copper has been demonstrated. Adaptive reorganization of the activity of enzymic systems (xanthine oxidase, ceruloplasmin, succinate dehydrogenase, aspartate and alanine aminotransferases) and gradual involvement of neurohumoral mechanisms of the sympathoadrenal and cholinoreactive systems provide for adaptation of some animals in molybdenum and copper-molybdenum biogeochemical provinces. In other sheep, under the same conditions, dystonic disturbances in the vegetative nervous systems are observed together with the development of molybdenum toxicosis.
 ...
PMID:[The enzymatic chemical mechanisms of adaptation]. 183 7

In a herd of cattle located in central Hungary, illness and subsequent death of cows was observed. The cause of these losses was molybdenum-induced secondary copper deficiency. The origin of the environmental molybdenum was used motor oil containing molybdenum bisulfide as an additive. This split motor oil polluted the cow's pasture located on the side of a railway bed near the farm. Before the illnesses and deaths, the animals were grazing for at least 2 weeks in the contaminated area. The ill animals were recumbent and unable to rise. There was no response to treatment with 10 g calcium gluconate iv. Cows which died showed no specific lesions on necropsy. The clinical chemistry investigations showed anemia, minimal caeruloplasmin activity in the blood, and high molybdenum concentrations in the rumen contents, liver and kidney. Copper concentrations were low in liver, kidney and blood serum. The seriously ill cows died in spite of 100 mg copper glycinate injections, but the asymptomatic animals remained alive. Molybdenum pollution can cause acute clinical disease and subsequent death by interfering with copper metabolism.
 ...
PMID:Secondary copper deficiency in cattle caused by molybdenum contamination of fodder: a case history. 271 4

The experiment comprised soil, herbage, and 3 groups of cattle from Cu deficient regions. Samples of blood, rumen contents, hair, urine and feces, while after slaughter, samples of the liver, kidney, brain, skeletal muscles and long bones were taken from animals under study. Low contents of copper, molybdenum, ceruloplasmin and lipid peroxides, approximately normal contents of zinc, iron, calcium, phosphorus, vitamin E and ascorbic acid, and increased concentration of sulphur were demonstrated. The highest activity of superoxide dismutase was found in the youngest animals.
 ...
PMID:Evaluation of selected metabolic indices for cattle in copper deficient regions. 350 18

The synthesis and purification of the sodium salts of di (MoO2S2(2-)), tri (MoOS3(2-)) and tetra (MoOS4(2-)) thiomolybdate is reported. All three compounds were reversible inhibitors of the ovine ceruloplasmin (Cp) catalysed oxidation of O-dianisidine. Na2MoO2S2 inhibited via a non-competitive mechanism whereas Na2MoOS3 showed mixed non competitive and Na2MoS4 competitive mechanisms. All showed upward curving slope replots. Molybdenum trisulfide (MoS3) was synthesized and displayed mixed type inhibition kinetics but with a linear slope replot. Preliminary evidence suggested that both MoOS3(2-) and MoS4(2-) may also be substrates for ovine Cp.
 ...
PMID:Reversible inhibition of ovine ceruloplasmin by thiomolybdates. 374 72

The O2-utilizing (type O, oxidase) form of xanthine oxidoreductase is primarily responsible for its ferroxidase activity. This form of xanthine oxidoreductase has 1000 times the ferroxidase activity of the serum ferroxidase caeruloplasmin. It has the ability to catalyse the oxidative incorporation of iron into transferrin at very low Fe2+ and O2 concentrations. Furthermore, the pH optimum of the ferroxidase activity of the enzyme is compatible with the conditions of pH that normally exist in the intestinal mucosa, where it has been proposed that xanthine oxidoreductase may facilitate the absorption of ionic iron. Modification of the molybdenum (Mb) centres of the enzyme in vitro by treatment with cyanide, methanol or allopurinol completely abolishes its ferroxidase activity. The feeding of dietary tungsten to rats, which prevents the incorporation of molybdenum into newly synthesized intestinal xanthine oxidoreductase, results in the progressive loss of the ferroxidase activity of intestinal-mucosa homogenates. Removal of the flavin centres from the enzyme also results in the complete loss of ferroxidase activity; however, the ferroxidase activity of the flavin-free form of the enzyme can be restored with artificial electron acceptors that interact with the molybdenum or non-haem iron centres. The presence of superoxide dismutase or catalase in the assay system results in little inhibition of the ferroxidase activity of xanthine oxidoreductase.
 ...
PMID:Studies of the ferroxidase activity of native and chemically modified xanthine oxidoreductase. 375 93

An attempt has been made to assess the importance of systemic sites of interaction from the effect of dietary molybdenum (Mo) on the protection afforded by a single sc injection of copper (Cu) to 29 initially hypocupraemic 5-year-old ewes, maintained on a low Cu diet. They were fed a diet of 1 kg/day containing 1.3 mg of Cu/kg supplemented with sodium sulphate which provided 1.7 gm of sodium per kg. Group A was given no further supplement. Group B was given added Mo, 25 mg/kg. Group C was given added Cu, 10 mg/kg. After 7 months, several animals in each group were injected sc with a single dose of 46.5 mg of Cu in the form of copper calcium edetate (Coprin). Blood samples were taken at intervals from the injected ewes over a 250-day period. All ewes were mated after 12 months on the diet. Injected ewes were approaching the 4th month of gestation when the last blood sample was taken at 250 days. Total Cu in plasma was determined by atomic absorption spectometry. Direct reacting Cu in plasma, cerulosplasmin oxidase activity, and hemoglobin were also estimated. Plasma Cu concentrations had increased to normal levels in 14 days in Group A after the Cu injections. Group B animals showed a greater increase, mean values exceeding those of Groups A and C, between Days 28-129 (p less than .01). Plasma Cu levels declined in ewes not given supplementary Cu after the 177th day. The final values for Groups A and B were similar to those found before injection. The direct reacting Cu in each group was increased after 7 days (p less than .05). This effect was most marked in the Mo supplemented ewes (Group B). The effect of Mo persisted until the final bleeding. Direct reacting Cu was only a minor part of the early response in total plasma copper of Group B ewes. Dietary Mo did not inhibit the incorporation of injected Cu into ceruloplasmin. The Mo-supplemented ewes were in poorer condition than copper-supplemented ewes. All groups gained in weight after the injections. The sc injection of Cu at 5 months prior to mating imporved fertility in Groups A and B. There was no evidence that dietary Mo impa ired the metabolism of parenteral Cu. However, it is known to deplete r uminants of Cu when the diet provides the only source of Cu. It is ther efore thought that the site of the Cu with Mo interaction is in the gut. If infertility due to Cu deficiency is suspected in a flock, an injection of Cu immediately prior to mating may improve conception rate and provide sufficient Cu to reduce the incidence of swayback.
 ...
PMID:The effect of dietary molybdenum on hypocupraemic ewes treated by subcutaneous copper. 444 11


1 2 3 4 5 Next >>