Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.16.3.1 (
ceruloplasmin
)
5,074
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Female rats were fed with ethanol extraction of Alismatis Rhizoma for 6 months to study its nephrotoxicity and molecular mechanism. HPLC was used to determine the components in ethanol extraction of Alismatis Rhizoma. An assessment of renal pathology was determined by HE staining. Meanwhile Western blot, immunohistochemical assay and q-PCR were used to assess the protein expression and mRNA level of Kim-1, clusterin,
LCN2
, osteopontin,
ceruloplasmin
and TIMP1 in rat kidney. Eight components were identified in ethanol extraction of Alismatis Rhizoma. Tubule-interstitial inflammation, renal tubular epithelial cell exfoliation and morphological changes of cell were observed in rat kidney. Comparing with control blank group, the protein expression of clusterin, Kim-1,
LCN2
, osteopontin and TIMP1 in rat kidney was significantly increased while the protein expression of
ceruloplasmin
was significantly decreased. The mRNA level of Kim-1, TIMP1, osteopontin, clusterin and
LCN2
was significantly increased while the mRNA level of
ceruloplasmin
was significantly decreased. In this study, it was inferred that there is chronic toxicity in kidney by using high dosage of ethanol extraction of Alismatis Rhizoma for a long time. And the underlying molecular mechanism was related to regulate the protein expression of
ceruloplasmin
, clusterin, Kim-1,
LCN2
, osteopontin and TIMP1.
...
PMID:[Study on nephrotoxicity of ethanol extract from Alismatis Rhizoma in rats and its molecular mechanism]. 2892 28
