Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.16.3.1 (ceruloplasmin)
 5,074 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The purification of the pregnancy zone protein by means of immunoadsorbents is described. The pregnancy zone protein antibody was isolated from an absorbed rabbit antiserum and coupled with CNBr-activated sepharose. The pregnancy zone protein was isolated from pregnancy serum by the specific antibody cross-linked with sepharose. Contaminating serum proteins were eliminated by "inverse" immunoadsorption using antibodies against these proteins coupled with sepharose. An immunoelectrophoretically pure pregnancy zone protein was obtained. By means of a combination of immunoprecipitation and enzyme reaction in agar gel could be excluded that the pregnancy zone protein possesses activities of the following 11 enzymes: ceruloplasmin, leucine amino peptidase, alkaline phosphatase, carboxylic esterase, lactate dehydrogenase, malate dehydrogenase, glycerophosphate dehydrogenase, glucose-6-phosphat-dehydrogenase, cholinesterase, acetyl cholinesterase and oxytocinase.
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PMID:[Isolation of "pregnancy-zone" proteins using immuno absorbents and study of possible enzyme activities]. 17 12

The purpose of this study was to investigate whether a high fat intake would potentiate an excess of fructose in the diet of rats to alter energy metabolism and worsen the nutritional status for copper. Weanling male rats were fed diets with 45% fat and 20% cornstarch, 45% fat and 20% fructose, 5% fat and 60% cornstarch, or 5% fat and 60% fructose for 3 wk, with either sufficient (+) or deficient (-) amounts of copper. Copper deficiency, as demonstrated by low plasma copper and ceruloplasmin, caused a decrease of liver, heart, and testes copper; a decline of liver and heart zinc; and an increase of hepatic iron. High-fat diets reduced liver glucose-6-phosphate dehydrogenase (G6PDH) and, to a lesser extent, glycerophosphate dehydrogenase (GPDH). The interaction between high-fat and high-fructose diets, superimposed on copper deficiency, resulted in a lowering of G6PDH, GPDH, and malic enzyme. These results support the hypothesis that a high fat ingestion becomes an aggravating stress factor, which, in combination with a high-fructose intake in a copper-deficient diet, adversely alters key organ mineral content, with detrimental effects on copper nutritional status and intermediary metabolism.
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PMID:Copper deficiency: interaction with high-fructose and high-fat diets in rats. 782 19