Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.14.99.3 (
heme oxygenase
)
4,196
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Upstream stimulatory factor (USF), originally identified in HeLa cells, interacts with the upstream promoter sequence of adenovirus 2 major late promoter (Ad2MLP) and activates its transcription. USF is present in uninfected HeLa cells and appears to be involved in the transcription of cellular genes related to stress. Recently, we have proposed that the rat
heme oxygenase
gene, newly identified heat-shock protein gene, is regulated at least in partly by a
rat homolog
of USF [Sato, M., Fukushi, Y., Ishizawa, S., Okinaga, S., Muller, R.M. & Shibahara, S. (1989) J. Biol. Chem. 264, 10251-10260]. We therefore confirm that the
heme oxygenase
gene is expressed in HeLa cells and its expression is increased by cadmium, suggesting that human
heme oxygenase
is a stress protein similar to the metallothioneins. Using partially purified USF from HeLa cells, we show that USF binds to the human
heme oxygenase
gene promoter and stimulates its cell-free transcription. The cis-acting element, identified as CACGTGACCCG, is located 34 bp upstream from the transcription initiation site, and contains the core sequence of the upstream promoter sequence of Ad2MLP. We propose that USF contributes to the transcription of the human
heme oxygenase
gene.
...
PMID:Interaction of upstream stimulatory factor with the human heme oxygenase gene promoter. 231 8
