Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.14.11.2 (
prolyl hydroxylase
)
1,814
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Cells that contain the high (
NF-H
) and medium (NF-M) molecular weight neurofilaments and neuron-specific enolase (NSE) have been cultured from human brain (Brodmann frontal area 9 and hippocampus) obtained at routine autopsy. Three of these cultures (from a 52-year-old man with vascular dementia, a 75-year-old man with Alzheimer's disease, and an 80-year-old normal woman) have been propagated to passage 12-14 and studied in detail. Cellular morphology was pleomorphic and consistent with a relatively low level of differentiation, by both light microscopic and ultrastructural examination. Immunocytochemical reactivity in a characteristic filamentous pattern was observed with monoclonal antibodies to nonphosphorylated epitopes of
NF-H
and NF-M as well as with redundant antibody probes specific for
NF-H
and NF-M. Immunoblotting studies confirmed the presence of
NF-H
and NF-M. Immunocytochemistry and Immunoblotting also demonstrated the presence of NSE, but not of glial fibrillary acidic protein or other glial markers. Immunocytochemical studies also indicated the presence of
proline hydroxylase
, an enzyme found in fibroblasts but not neurons. These studies indicate that cells can be cultured from autopsy human brain which are not neurons but nevertheless express molecules used as markers (
NF-H
, NF-M, and NSE) of adult neurons in vivo and in culture.
...
PMID:Presence of neuronal proteins in serially cultured cells from autopsy human brain. 815 4
