Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.14.11.2 (
prolyl hydroxylase
)
1,814
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Adherent cells derived from human palatine tonsils were isolated and cultivated. Exponentially growing adherent cells (
TAC
) were observed by phase-contrast microscopy and transmission electron microscopy. Immunocytochemical studies were also performed.
TAC
were composed of relatively monotonous cells with polygonal or spindle shapes and high proliferative activity. In addition to the development of rough endoplasmic reticulum and lysosomes, the
TAC
possessed a moderate amount of pinocytotic vesicles and a few microfilaments. All of the
TAC
strongly expressed fibroblastic markers and partial monocyte/macrophage markers, such as beta-subunit of
prolyl 4-hydroxylase
(DAKO-fibroblast), lysozyme, anti-alpha-1-antichymotrypsin (alpha ACT), and CD68 (KP-1, EBM/11). It was noted that, as the
TAC
were cultured for a longer period, they gradually increased the reactivity with the monoclonal antibody PG-M1. Furthermore, the
TAC
expressed myocytic phenotype, such as alpha-smooth muscle actin (alpha SMA) with various intensity. Moreover, as to extracellular matrix,
TAC
stained for collagen type I, collagen type III, laminin, and fibronectin. Collagen type IV was weakly positive. The results presented here showed that the
TAC
expressed three different phenotypes of fibroblasts, histiocytes and smooth muscle cells at the same time. The monoclonal antibody raised against the
TAC
reacted strongly with the subendothelial pericytes and/or smooth muscle cells in the extrafollicular area in human tonsils. The present results also suggested that the origin of the
TAC
was probably subendothelial pericytes and/or smooth muscle cells of the microvasculatures in the tonsil.
...
PMID:Co-expression of fibroblastic, histiocytic and smooth muscle cell phenotypes on cultured adherent cells derived from human palatine tonsils: a morphological and immunocytochemical study. 880 95
