Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.13.12.5 (
aequorin
)
1,451
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The effect of some general and local anaesthetics on the calcium-induced luminescence of
aequorin
was studied in vitro using a photomultiplier tube and recording technique. Purified
aequorin
(0.1 microliter) was injected into a 500 micron diameter porous cellulose acetate capillary tube containing 0.5 M KC1, 20 mM phosphate (pH 7.2) and calcium-EGTA buffers. The trapped
aequorin
was superfused with buffer solutions which sometimes contained anaesthetic (test) solutions. The results showed that some anaesthetics, e.g. urethane, etomidate and lignocaine, increased whereas others, e.g. methohexitone, thiopentone, decreased the light output (luminescence) of
aequorin
in constant ionized calcium and EGTA buffers. Similar results were produced by some non-anaesthetic drugs, e.g. glycerol,
TEA
, caffeine, etc. Concentration-response curves for calcium-dependent and -independent luminescence of
aequorin
showed that anaesthetics variously affected the
aequorin
response. Some anaesthetics, e.g. lignocaine, increased the maximum response while others, e.g. etomidate, increased the affinity (i.e. decreased EC50s) of
aequorin
to calcium ions without altering the slope, which remained at about 2. It was concluded that anaesthetics can either excite or depress
aequorin
luminescence, the effect being dependent on the type and the concentration used.
...
PMID:Effect of anaesthetics on calcium-induced luminescence of aequorin. 286 59
