Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.12.7.2 (
hydrogenase
)
3,522
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Di-myo-inositol-1,1(prm1)-phosphate (
DIP
) is present at a significant concentration ((symbl)160 nmol/mg of protein) in the cytoplasm of the hyperthermophilic bacterium Thermotoga maritima. The concentration of
DIP
was independent of the pH of the growth medium or the cell growth phase but increased with increasing concentrations of NaCl in the growth medium, reaching a maximum ((symbl)450 nmol/mg of protein) at 0.4 to 0.6 M NaCl. A large-scale purification procedure for
DIP
which yields approximately 18 g of
DIP
per kg of cells (wet weight) is described. Purified
DIP
was stable at 90(deg)C for at least 5 h. The presence of
DIP
(50 mM) did not increase the stability at 90(deg)C of pure forms of the
hydrogenase
or pyruvate ferredoxin oxidoreductase of T. maritima, suggesting that
DIP
is not a general thermoprotectant.
...
PMID:Characterization of Di-myo-Inositol-1,1(prm1)-Phosphate in the Hyperthermophilic Bacterium Thermotoga maritima. 1653 98
