Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.12.7.2 (hydrogenase)
 3,522 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cosmid 1 containing the hup genes isolated from the photosynthetic bacterium Rhodobacter sphaeroides was studied. The hupR gene from cosmid 1 was cloned and sequenced (EMBL accession number AJ243734). It encoded a 54.031 kD protein homologous to transcriptional regulators belonging to the superfamily of two-component regulatory systems. The HupR protein was overexpressed in Escherichia coli in the form of His6-tagged HupR. The cloned hupR gene could restore hydrogenase activity in R.sphaeroides hupR mutants and activate hupSL gene transcription.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2001
PMID:Isolation and Analysis of hupR Gene Required for the Expression of Hydrogenase in Rhodobacter sphaeroides. 1203 50

HupT(-) insertion mutants, SDHT1 and SDHT2, were constructed by the homologous double exchange between the host, Rodobacter sp. SDH20, and the hupT gene fragment with a kan(R) gene inserted on pSE8, a suicide plasmid, which had been introduced into the host by using triparental conjugation. The HupT(-) mutants were verified by Southern hybridization. H(2)-uptake hydrogenase activity analysis revealed that the H(2)-uptake hydrogenase activities of HupT(-)mutants were two-fold as that of wild type strain SDH20 when grown anaerobically in MN medium and aerobically in MG or MN medium, and had no distinct improvement relative to wild type strain when grown anaerobically in MG medium. The expression analysis of hupS confirmed that the HupT- mutants exhibited two- to three-fold increase of the expression of hupS relative to wild type strain when grown anaerobically in MN medium and aerobically in MG or MN medium, but no obvious increase when grown anaerobically in MG medium. These results demonstrate that hupT regulates negatively the synthesis of H(2)-up-take hydrogenase in the photosynthetic bacterium strain SDH2.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 1999
PMID:Mutagenesis of hupT Gene and H(2)-uptake Hydrogenase Expression in Photosynthetic Bacterium SDH2. 1213 74