Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.12.7.2 (
hydrogenase
)
3,522
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Several accessory proteins are required for the assembly of the metal centers in hydrogenases. In NiFe-hydrogenases, CO and CN
-
are coordinated to the Fe in the NiFe dinuclear cluster of the active center. Though these diatomic ligands are biosynthesized enzymatically, detail mechanisms of their biosynthesis remain unclear. Here, we report the structural characterization of HypX responsible for CO biosynthesis to assemble the active site of NiFe
hydrogenase
.
CoA
is constitutionally bound in HypX. Structural characterization of HypX suggests that the formyl-group transfer will take place from N
10
-formyl-THF to
CoA
to form formyl-
CoA
in the N-terminal domain of HypX, followed by decarbonylation of formyl-
CoA
to produce CO in the C-terminal domain though the direct experimental results are not available yet. The conformation of
CoA
accommodated in the continuous cavity connecting the N- and C-terminal domains will interconvert between the extended and the folded conformations for HypX catalysis.
...
PMID:Structural characterization of HypX responsible for CO biosynthesis in the maturation of NiFe-hydrogenase. 3164 88
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