Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.12.7.2 (
hydrogenase
)
3,522
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Escherichia coli DNA was digested with restriction endonuclease PstI and ligated into the PstI site of plasmid pBR322. Recombinant plasmids that were constructed in this manner were used to transform E. coli H61, a mutant with a decreased level of
hydrogenase
activity. Complementation of this
hydrogenase
mutation identified a bacterial clone carrying the gene for the membrane-associated E. coli
hydrogenase
in plasmid pBL101. In E. coli minicells, the pBL101
DNA directed
the synthesis of a protein of a size corresponding to that of the precursor of the E. coli membrane-associated
hydrogenase
, which appears to contain an uncleaved leader peptide. A restriction map of the cloned DNA was determined for 14 endonucleases.
...
PMID:The identification and partial characterization of a plasmid containing the gene for the membrane-associated hydrogenase from E. coli. 627 43
