Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.12.7.2 (hydrogenase)
 3,522 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The metabolism of [4-14C]progesterone in the parotid salivary glands of nonpregnant female, pregnant female and male rats were investigated in vitro. The metabolic activity of the male rats was significantly lower than in either of the female groups. The pregnant group was metabolically more active than the nonpregnant female group, but his differences was not statistically significant. I homogenates and soluble fractions the main metabolite was 20-alpha-hydroxy- 4-pregnen-3-one in female rats. In male rats the main metabolites were 20-alpha-hydroxy-4- pregnen-3-one and 3-alpha-hydroxy-5-alpha-pregnan-20-one in homogenates and 20-alpha-hydroxy-4- pregnen-3-one in soluble fractions. In the microsomal fractions of both sexes polar compounds predominated. The results indicated the presence of at least the following progesterone metabolizing enzymes in art parotid salivary glands; 3-alpha-, 3-beta-, 20-alpha- and 20-beta-hydroxysteroid dehydrogenase, 5-alpha-and 5-beta-steroid hydrogenase and 17-alpha-steroid hydroxylase activities. Ind the homogenates and soluble fractions of female rats 20-alpha-hydroxysteroid dehydrogenase activity was significantly higher than in males.
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PMID:Progesterone metabolism by major salivary glands of rat--II. Parotid gland. 227 46

The metabolism of progesterone by the submandibular and sublingual salivary glands of female (nonpregnant and pregnant) and male rats was studied. The metabolism was in both sexes significantly greater in submandibular than in sublingual glands. Sex differences were not seen in sublingual glands but less metabolism was found in homogenates and microsomal fractions of female (nonpregnant and pregnant) submandibular glands compared to that of males. The metabolism did not differ between pregnant and nonpregnant female rats. The metabolites were mainly 5 alpha-pregnane-compounds. On the basis of the metabolites identified it can be concluded that rat submandibular and sublingual glands contain at least 3 alpha-, 3 beta-, 20 alpha- and 20 beta-hydroxysteroid dehydrogenase, 5 alpha- and 5 beta-steroid hydrogenase and 17 alpha-steroid hydroxylase activity. 5 alpha-steroid hydrogenase activity was significantly higher in all preparations of male submandibular glands than in females. In sublingual glands some enzyme activities showed pregnancy-related decreased.
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PMID:Progesterone metabolism by major salivary glands of rat--I. Submandibular and sublingual glands. 236 33

[4-14C]Progesterone was incubated with homogenate and mitochondrial, microsomal and soluble fraction preparations of healthy and inflamed gingiva from human subjects of both sexes. The subcellular preparations were supplemented with an NADPH-regenerating system and incubated for 2 h at pH 7.4 and 37 degrees C. The metabolites were identified by column, multiple TLC and radioautography and quantified with liquid scintillation counting. In inflamed tissue the metabolic activity was higher than in healthy gingiva. On the basis of the identified metabolites it can be concluded that the human gingiva of both sexes contains marked 3 alpha-, 3 beta- and 20 alpha-hydroxysteroid dehydrogenase, delta 4-5 alpha- and delta 4-5 beta-steroid hydrogenase activities, and less 20 beta-hydroxysteroid dehydrogenase activity.
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PMID:Metabolism of progesterone by healthy and inflamed human gingiva in vitro. 409 11