Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:1.11.1.7 (peroxidase)
65,474 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Parotid glands from adult dogs were stained with a battery of seven horseradish peroxidase-conjugated lectins (PNA, UEA, LTA, DBA, SBA, WGA and ConA). In some cases (PNA and DBA) neuraminidase digestion was followed by lectin staining. Acinar cells contained conspicuous quantities of oligosaccharides with terminal sialic acid radicals. Galactosil-(beta 1-->3) N-acetylgalactosamine was the most abundant penultimate sugar linked to N-acetylneuraminic acid. Sialylated components having the terminal dimer sialic acid-N-acetylgalactosamine were found in the acinar cells. Secretory cells presented a heterogeneous distribution of glycoconjugates with terminal fucose and beta-N-acetylgalactosamine. Fucose, N-acetylglucosamine and alpha-N-acetylgalactosamine were present on the apical cytoplasm and surface of the striated and interlobular duct cells. This glycosidic composition was unaffected by extensive selective breeding. The role of abundant amounts of sialic acid radicals in the oral mucosa was considered.
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PMID:Localization of glycoconjugates in dog parotid gland by lectin histochemistry. 783 56

5,8,11,14-eicosatetraynoic acid (ETYA), an isomorphic competitive analogue of arachidonic acid, spontaneously generates a chemiluminescence signal detected with a liquid scintillation spectrometer operated at ambient temperature in the out-of-coincidence mode. The intensity of the signal was 10- or more-fold above background, required oxygen for its generation, was inhibited by antioxidants, and approximately doubled in D2O. Arachidonic acid, which contains 4-alkene rather than alkyne bonds did no more than double the chemiluminescent signal above background. When examined at 37 degrees C in a Berthold AutoLumat 958 luminometer, DBA (lucigenin) was required to detect a signal above background. Catalase or peroxidase, and to a lesser extent mannitol or histidine but not superoxide dismutase, strongly diminished the signal intensity. These observations provide a baseline for interpreting the functional and electron microscopic changes produced by ETYA in PC3 prostate and A172 glioblastoma cell lines, consistent with a contribution from oxidative stress associated with free radicals, and the absence of these morphological changes in U937 monoblastoid cells.
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PMID:Spontaneous chemiluminescence of ETYA (5,8,11,14-eicosatetraynoic acid) is inhibited by catalase or peroxidase. 784 95

The present investigation was undertaken to assess the binding characteristics of eight different lectins to normal oral mucosa (11 cases), leukoplakia with varying degrees of dysplasia (five cases), and oral mucosal squamous cell carcinoma (12 cases) by the use of biotinylated lectins and avidin biotin peroxidase complexes. The lectins employed were soybean (SBA), peanut (PNA), Dolichos biflorus (DBA), concanavalin A (Con A), wheat germ (WGA), Ulex europaeus (UEA), Ricinus communis (RCA), and Lotus tetragonolobus (LTA). It was observed that SBA, DBA, WGA, UEA, RCA, and LTA showed very strong to strong binding in healthy oral mucosa but no or very weak binding in squamous cell carcinoma. On the contrary, PNA showed weak binding to normal mucosal epithelial cells but showed strong binding to malignant cells. The dysplastic mucosa had an intermediate binding pattern. The lectin Con A was not bound at all or seen in very low concentration in the malignant cells and dysplastic epithelium, but it showed weak binding in the normal mucosa. Hence, we conclude that lectins may be utilized as probes to determine the dysplastic and malignant status of the oral mucosal epithelium.
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PMID:Binding pattern of eight different lectins in healthy subjects and patients with dysplastic and malignant lesions of the oral cavity. 824 72

Lectin binding in normal and irradiated embryonic mouse tissues on day 10 of gestation was studied by peroxidase techniques. Specific binding of Dolichos biflorus lectin (DBA) was detected in the mesodermal blood vessels and in the otic vesicles. The amount of DBA as well as that of soybean agglutinin (SBA) and peanut agglutinin (PNA) increased after exposure to low doses of radiation (0.25, 0.50 and 0.75 Gy). The modifying influence of ionizing radiation was observed in the pituitary region, in the otic vesicles and in the blood vessel endothelium. The greatest effect appeared in the pituitary region at 0.75 Gy, while in the otic vesicles it appeared at 0.50 Gy. A dose-effect relationship was established for the DBA lectin affinity of the vascular endothelium. In comparison to DBA, SBA and PNA displayed more extensive staining after irradiation. The reactivity of these lectins appeared especially pronounced on the blood vessels within the central nervous system and in the luminal surface of the ependymal cells. It is of interest that maximal binding for PNA was observed at 0.25 Gy and for SBA at 0.50 Gy at the junctions between neuroepithelial cells.
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PMID:Histochemical investigations on lectin binding in normal and irradiated mouse embryos. 833 57

Fragments of metanephros were taken from chick embryos and studied from the 7th to the 21st d of incubation. A battery of 7 different horseradish peroxidase-labelled lectins (PNA, ConA, DBA, SBA, LTA, WGA and UEA I) was used to analyse the distribution and changes of carbohydrate moieties in glycoconjugates along the metanephric nephron from the S-shaped body stage onwards. DBA and SBA reacted for a short time at some sites during the considered period of incubation. Provided WGA and ConA reacted with most of the nephric components, the almost ubiquitous presence of N-acetyl-D-glucosamine and alpha-D-mannose was demonstrated. SBA and LTA were found to be good markers of the proximal tubule. On the 12th d of incubation, sialic acid was found in the podocytes, capillary walls, and the connecting and collecting ducts. On the same day, other oligosaccharides were present at the nephric tubular components. The simultaneous presence of sialic acid and other sugar residues may be significant for the identification of the exact stage of incubation at which metanephric activity starts. The possible role of some sugar residues for the regulation of metanephric activity is discussed.
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PMID:Chick embryo metanephros: the glycosylation pattern as revealed with lectin conjugates. 835 73

The effect of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) on the mucin phenotype of non-metaplastic gastric mucosa in the rat was studied histochemically. Animals were exposed to MNNG in drinking water (83 mg/l) for 12 weeks. Carcinogen treatment was then discontinued and the animals (27 in the treatment group and 25 in the control group) were examined after another 44 weeks. Glycosylation was analysed with histochemical stains for sialomucins and sulphomucins and with peroxidase-conjugated lectins (GS-II, SBA, DBA, UEA-I, and WGA). Sialo- and sulphomucins remained quantitatively unchanged, only a slight increase of acid mucins in the antral glands was observed. The analysis of the lectin binding patterns, however, revealed a significant increase for WGA-binding glycoproteins in the surface mucous cells and gastric pits, while DBA binding was significantly decreased (P < 0.05). GS-II lectin bound specifically to the proliferative compartment in the gastric fundus, consisting of mucous neck cells, and was significantly increased after MNNG treatment. No specific alterations were detected in lectin binding to parietal or chief cells. It is concluded, therefore, that treatment of gastric mucosa with MNNG alters the glycoprotein metabolism before intestinal metaplasia can be observed.
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PMID:Effect of N-methyl-N'-nitro-N-nitrosoguanidine on carbohydrate profiles of non-metaplastic rat gastric mucosa. 841 88

The distribution of lectin receptors in the human tonsil was studied using 16 biotinylated lectins. The avidin-biotin-peroxidase complex (ABC) method was used on frozen and paraffin-embedded tissue sections. Cell suspensions were also analysed by dual flow cytometry using respective fluorescein isothiocyanate-conjugated lectins and phycoerythrin-labeled anti-CD3 and anti-human immunoglobulin. Frozen sections fixed with acetone and paraffin-embedded materials fixed in three solutions were compared for lectin affinity; ethanol-fixed sections gave best results followed by frozen and buffered formalin-fixed ones, then nonbuffered formalin. Con-A, RCA-1, LcH, WGA, MPA, PHA, PSA, PNA, SJA and GSA-1 reacted with all tissue components of the tonsil in immunohistochemical studies, but binding intensity was fixative dependent. Binding of Lotus and BPA to lymphocytes was limited to germinal center lymphocytes. Other tissue components were also reactive but staining intensity was weaker in Lotus compared with BPA. SBA and DBA did not react with lymphocytes, but reacted with macrophages/histiocytes, vascular endothelia, and epithelial cells. LBA and LPA were constantly negative with all tissue components irrespective of fixatives. Flow cytometric analyses showed that all but three (DBA, LBA and LPA) partially or totally stained lymphocyte surfaces. Lotus receptors were expressed exclusively on B-lymphocytes.
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PMID:Distribution of lectin receptors in the human hyperplastic tonsil: histochemical and flow cytometric analyses. 846 May 51

Gamete transport, fertilization, and early embryonic development take place in different regions of the oviduct and under different hormonal conditions. The objective of this study was to use lectins to detect variation in the distribution of glycosylated molecules on the surface of the epithelia that influence these events. Oviducts were collected from gilts on day 1 (estrus) and day 16 (diestrus) of the estrous cycle, and from gilts that were bilaterally ovariectomized on day 4 of the estrous cycle, and were subsequently treated with estradiol valerate (100 micrograms/day), progesterone (200 mg/day), or corn oil vehicle for 11 days. Six biotinylated lectins, Triticum vulgaris (WGA), Arachis hypogaea (PNA), Ulex europeus (UEA-I), Dolichos biflorus (DBA), Ricinus communis (RCA-I), and Canavalia ensiformis (Con A), were used to probe tissue from the isthmus and ampulla using peroxidase-conjugated avidin as the marker. In cyclic gilts, WGA and DBA stained more strongly in the isthmus than the ampulla on both cycle days. Staining with PNA was patchy, but greater on the apical surface of isthmic epithelium on day 16 than day 1. With UEA-I, staining was more intense in the ampulla on day 16 than day 1. Staining by PNA and UEA-I was suppressed in both the ampulla and isthmus with estradiol treatment. DBA staining was suppressed in the isthmus with both estradiol and progesterone treatments. Cilia were labelled by all lectins except WGA and PNA. Thus glycosylation patterns vary in the porcine oviduct with region and hormonal state, reflecting the variety of events that may be influenced.
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PMID:Distribution of lectin binding sites in the oviducts of cycling and hormone-treated pigs. 848 17

The development of the esophagus in the chick embryo is characterized by remarkable morphological changes especially at the level of the epithelium. Using horseradish peroxidase-conjugated lectins (DBA, PNA, SBA, WGA, ConA, LTA, UEAI) we have studied, at the level of the esophagus of chick embryos from the 8th to the 21st day of incubation and of 1- and 2-day-old chicken, the evolution of the saccharidic moieties of glycoconjugates, which precedes and/or is concomitant with the epithelial morphological transformations. We have found differences in content and cellular distribution of oligosaccharides during the histogenetic processes which characterize the lining and glandular epithelium. Before the appearance of cilia and mucus secretion at the bathyprismatic epithelial cells, the sugar residues D-galactose-(beta 1-->3)-N-acetyl-D-galactosamine, beta-N-acetyl-D-galactosamine and alpha-L-fucose were detected only at the luminal cell surface. These oligosaccharides were probably involved in giving rise to the polarization of the esophageal epithelial cells. The esophageal gland mucus was first characterized by the presence of alpha-L-fucose and afterwards also by the presence of D-galactose-(beta 1-->3)-N-acetyl-D-galactosamine, D-glucosamine and sialic acid.
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PMID:Characterization of the glycoconjugate sugar residues in developing chick esophageal epithelium. 849 Feb 63

Seven lectins (PNA, DBA, SBA, UEA I, LTA, WGA and ConA), conjugated with horseradish peroxidase, were used to characterize the glycosidic residues in the zygomatic gland of adult dogs. In some cases (PNA and DBA), lectin staining was preceded by neuraminidase digestion. The acinar and tubular cells produced glycoconjugates with different sugar residues, presenting binding sits for all of the lectins used. The apical surfaces of the cells lining the intra- and interlobular ducts were also stained by all the lectins. In contrast, the demilunar cells only reacted with the Neu-PNA sequence and Con A.
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PMID:A lectin histochemical study of the zygomatic salivary gland of adult dogs. 856 Jul 53


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