Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.11.1.7 (peroxidase)
 65,474 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Routine hematological tests were performed in a family which was at risk for sickle cell disease. Cellulose acetate electrophoresis and Triton PAGE were employed to differentiate between various variants of hemoglobin. Based on the data a pedigree was constructed which indicated that few members of the respectively had received the S gene, some of them were sickle cell disease while few were sickle cell trait. Elevated levels of peroxidase enzyme in affected individuals reflect its involvement in RBCs destruction. Statistical analysis strengthen this statement.
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PMID:Pedigree analysis and involvement of peroxidase in sickle cell disease. 133 54

Cellulose acetate membrane on which proteins are separated by two-dimensional electrophoresis and visualized by Coomassie staining is directly subjected to an enzyme-linked immunodetection method using a horseradish peroxidase-conjugated second antibody. The Coomassie-stained and immunochemically stained spots are distinguishable by their colors, such as blue with Coomassie blue staining and purple with horseradish peroxidase staining. Two kinds of lighting make the distinction more apparent. Coomassie-stained protein patterns are clear in the transmitted light. However, the immunochemically stained protein spots are obvious in the reflected light, in which Coomassie-stained patterns are obscure. The procedures do not require proteins to transfer onto nitrocellulose paper in contrast to proteins in polyacrylamide gel. The simple procedure excludes the nonspecific binding of the first and second antibodies to blocking proteins such as bovine serum albumin or gelatin because blocking protein is not used. With this method, matching of the Coomassie blue-stained spot with the immunochemically stained spot is done accurately and easily.
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PMID:Enzyme-linked immunodetection of proteins on Coomassie blue-stained two-dimensional cellulose acetate membranes. 354 52

Cellulose acetate (CA) discs placed in the peritoneum of mice become coated by a layer of peritoneal cells consisting primarily of macrophages (M). These CAM support the growth of hematopoietic colonies when syngeneic bone marrow cells are injected intraperitoneally. Most of these colonies are granulocytic and are recognizable by their peroxidase reaction. Since silica and carrageenan are known to reduce macrophage function, their effect on CAM granulocyte colony formation was tested. Carrageenan injected intraperitoneally before, concurrently, or after injection of marrow cells markedly reduced colony formation. Silica injected intraperitoneally concurrently or after injection of marrow cells reduced colony formation. Silica injected before marrow cells did not reduce colony formation. CAM produced in one mouse and exposed to carrageenan or silica in situ for 24 h before being transferred to sublethally irradiated recipients and seeded by injection of marrow cells had control levels of granulocytic colonies. Likewise, CAM produced in one mouse, removed, incubated in vitro with carrageenan or silica, carefully rinsed and transferred to sublethally irradiated recipients and seeded with marrow cells were able to support control levels of colony formation. Intravenous injection of silica or carrageenan had no consistent effect on colony formation. Spleen colonies (CFU-S) from marrow cells incubated in vitro with the agents, and given intravenously to lethally irradiated mice, were inhibited by silica, but not by carrageenan. Silica or carrageenan given intravenously to irradiated mice 3 h before or 3 h after intravenous marrow cell injection enhanced subsequent CFU-S formation.
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PMID:Influence of silica and carrageenan on spleen colonies and colonies in murine peritoneal cell-coated cellulose acetate membranes. 629 99