Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:1.11.1.7 (peroxidase)
65,474 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Tyrosine hydroxylase, dopa oxidase, and peroxidase activities were studied in soluble fractions of B16 melanoma tumor homogenates by polyacrylamide gel disc electrophoresis. Stained gels were scanned photometrically and gel slices were assayed radiometrically. In these preparations, the two bands of tyrosine hydroxylating activity were completely separated from the peroxidase activity but coincided with two major bands of dopa oxidase activity. The third dopa oxidase band coincided with the single band of peroxidase activity. The soluble fraction of cultured cell homogenates had no peroxidase activity, but the two tyrosine hydroxylase bands coincided exactly with the two dopa oxidase bands. Therefore, in the soluble fraction of the murine melanoma bifunctional tyrosinase does exist as two electrophoretically separable forms which are independent of peroxidase.
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PMID:Characteristics of tyrosinase in B16 melanoma. 1 62

A transplantable myelogenous leukemia of an inbred Wistar/Furth rat has been established in tissue culture and cloned. The resulting transplantable leukemia line demonstrates in vitro doubling time of 20 hr, colony-forming efficiency of 5% in liquid and methylcellulos-containing medium, and a saturation density of 3.0 x 106 cells/sq cm in liquid medium. Following intraperitoneal inoculation, newborn rats developed solid tumors, ascities, and leukemia with ld50 of5 x 103 cells and mean latency of 60 days. The tumor cell morphology was consistent with that of acute myelogenous leukemia. Histochemical staining for myeloid enzymes revealed no evidence of myeloperoxidase, esterase, or leukocyte alkaline phosphatase; however, fluorescent antibody staining for lysozyme was markedly positive. Serum, urine, and ascitic fluid from rats with transplanted leukemia also contained elevated levels of lysozyme. There was no detectable type-CRNA virus production by this cell line after as long as 100 days in vitro. This inbred rat myelogenous leukemia should provide a useful model for studies of chemotherapy and immunoltherapy of human acute myelogenous leukemia.
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PMID:Acute myelogenous leukemia of the Wistar/Furth rat: establishment of a continuous tissue culture line producing lysozyme in vitro and in vivo. 4 87

None of the radionuclides with which bleomycin has been labeled have chemical and nuclear properties that are entirely satisfactory for in vivo tumor localization. Bleomycin has been radioiodinated by the iodine monochloride, chloramine-T, and lactoperoxidase methods. Iodine monochloride proved to be the preferred method and conditions were developed whereby 80% of radioiodide was covalently bound to bleomycin. Bleomycin (140 mug) was added to 200 mul of saline/citrate buffer (pH 7.0) followed by radioiodide and iodine monochloride. This reaction mixture was incubated for 1 hr and purified by Sephadex G-10 chromatography. The iodine monochloride reaction product underwent hydrolytic deiodination in vitro at a rate of about 1.2%/day (0.15 M NaCl, 37 degrees C). Bleomycin A and B components were radioiodinated with equal efficiency on a mole fraction basis.
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PMID:Preparation and chemical characterization of radioiodinated bleomycin. 5 Oct 79

A method is reported for the preparation of glutaraldehyde cross-linked horseradish peroxidase conjugates for the visualization of concanavalin A binding to frozen sections. The binding of concanavalin A to tissue sections of human colonic tumor is used to compare staining by horseradish peroxidase conjugates and monomers. Without decreasing specificity, the use of progressively larger molecular weight conjugates provides progressively greater intensity of staining of the epithelial components of colonic carcinomas and normal colons.
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PMID:The use of glutaraldehyde-conjugated horseradish peroxidase-bovine serum albumin in the visualization of concanavalin A binding to tissue sections of human colonic tumor. 6 92

An experimental procedure for detecting and characterizing tumor-associated, virion, and histocompatibility antigens has been developed. The method takes advantage of the high resolution that proteins, solubilized by Triton X-100 and reduced, display after sodium dodecyl sulfate gel electrophoresis. The antigens can be detected as distinct molecular weight species by a highly sensitive inhibition of cytotoxic reaction. When coupled to the lactoperoxidase-catalyzed iodination of intact cells, the procedure permits the determination of externally exposed antigens. In the present study, the method has been applied to the Moloney leukemia virus-induced YAC lymphoma cells of strain A mice, which express a Moloney leukemia virus-determined cell surface antigen (MCSA) in addition to the type C viral proteins gp71, p30, p15, p15(E), p12, and p10. MCSA was identified as an exposed surface protein distinct in size and antigenic determinants from the major envelope and core protein of Moloney leukemia virus and the histocompatibility antigens. Multiple molecular weight species possessing antigenic determinants for MCSA, gp71, and H-2(a) have been detected. These results provide direct confirmation that MCSA is unrelated to the known virion structural proteins or to the H-2(a) antigen. This method should permit the direct identification and molecular weight characterization of any antigen whose determinants are not solely dependent on a complex quaternary structure and for which serological reagents are available.
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PMID:Moloney leukemia virus-induced cell surface antigen: detection and characterization in sodium dodecyl sulfate gels. 7 31

Twenty-two pituitary adenomas in Cushing's disease were removed by transsphenoidal surgery. In six patients the pituitary tumor had become manifest following adrenalectomy (Nelson's syndrome). Sixteen tumors were microadenomas measuring from 2 to 9 mm, while two were diffuse invasive adenomas verified at postmortem examination. Light microscopy showed that the tumors were made of basophillic cells containing PAS-positive granules that stained blue with Herlant tetrachrome and lead hematoxylin. Immunocytochemical studies showed that the granules stained positively with antiserum to adrenocorticotrophic hormone (ACTH) or to beta-lipotropic hormone (beta-LPH) and the peroxidase-antiperoxidase complex. Electron microscopic study of the tumor cells showed ACTH and beta-LPH containing granules varying in size, shape, and amount. Perinuclear bundles of 70 A microfilaments constituted a specific ultrastructural finding.
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PMID:Pituitary adenomas in Cushing's disease. A histologic, ultrastructural, and immunocytochemical study. 8 Feb 6

An immunocytochemical and ultrastructural study using the Fab fragment of an anti-human Ig antibody labelled with peroxidase was carried out on affected lymph nodes from five Hodgkin's disease patients. The tumor cells (Reed-Sternberg cells and Hodgkin cells) showed an exclusively hyaloplasmic granular staining. By comparing these grains with ribisome staining. By comparing these grains with ribosome staining of the endoplasmic reticulum of plasma cells it could be suggested that they are free risobomes. This ribosomal Ig synthesis is a major argument for the B lymphocyte nature of Reed-Sternberg and Hodgkin cells. The total absence of vacuole staining allows us to conclude that these cells are not histiocytic or macrophage derivatives.
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PMID:Ultrastructural and immunocytochemical localization of immunoglobulin synthesis in tumor cells in Hodgkin's disease. 8 23

The ability of inflammatory tissue macrophages harvested on glass coverslips implanted in the s.c. tissue of C57BL/6 mice to kill tumor cells in vitro has been examined. Macrophage present on coverslips implanted for less than 4 days are devoid of spontaneous tumoricidal activity but can be rendered cytotoxic for syngeneic and allogeneic tumor cells in vitro by incubation in vitro with lymphokines release by mitogen-stimulated lymphocytes. Inflammatory macrophages on coverslips implanted for 4 to 7 days show significant spontaneous cytotoxicity for tumor cells in vitro, and their tumoricidal activity is further increased by additional incubation in vitro with lymphokines. With progression, the inflammatory macrophages harvested on coverslips implanted for longer than 7 days lack spontaneous cytotoxic activity and are also resistant to activation by lymphokines in vitro. These alterations in tumoricidal activity and responsiveness to lymphokines are accompanied by a marked reduction in the number of peroxidase-positive macrophages within the population, suggesting that maintenance of tumoricidal activity requires continuous influx of new peroxidase-positive macrophages from the circulation. Previously activated macrophages which have lost their tumoricidal activity and become refractory to reactivation by lymphokines in the extracellular environment can be reactivated by treatment in vitro with liposomes containing encapsulated lymphokines.
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PMID:Rapid decay of tumoricidal activity and loss of responsiveness to lymphokines in inflammatory macrophages. 8 67

A method is described whereby commercially available radioimmunoassay-grade antibodies specific for the polypeptide hormones calcitonin, gastrin, glucagon, and somotastatin are used to detect these antigens on paraffin sections of routinely fixed tissue. The hormone antibodies are applied to deparaffinized tissue sections as the primary specific immune sera using the standard peroxidase technic. The use of these hormone antibodies to detect their respective antigens has proved valuable in demonstrating polypeptide forming tumor cells in pathologic specimens.
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PMID:The utilization of radioimmunoassay antibodies for the immunohistologic staining of polypeptide hormones on paraffin-embedded tissue. 8 76

A variety of antigens may be detected in the serum of patients with hepatocellular carcinoma (HCC). The incidence and distribution of five antigens in 37 HCC and their relation to each other in a given tumor was examined by the peroxidase-antiperoxidase technique using formalin-fixed paraffin-embedded tissues. alpha 1-Antitrypsin was frequently expressed in HCC (73 per cent of cases), whereas alpha-fetoprotein and carcinoembryonic antigen were less common. HBsAg, but not HBcAg, was observed in tumor cells in seven of nine HCC from HBsAg-positive patients. In 20 HCC (54 per cent), two or more antigens, most frequently alpha 1-antitrypsin and alpha-fetoprotein, were detected. Double staining for simultaneous localization of two antigens in the same tissue section revealed that different antigens were usually present in different tumor cells, although some cells displayed two antigens simultaneously. These findings suggest that hepatocellular carcinoma cells are functionally heterogeneous, even if they appear histologically monomorphic.
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PMID:Distribution of five antigens in hepatocellular carcinoma. 8 43


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