Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:1.11.1.6 (catalase)
 55,569 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The characteristics of an unclassified Mycobacterium sp. isolated from three patients with Crohn's disease are presented. The organism is extremely fastidious and mycobactin dependent and may require up to 18 months of incubation for primary isolation. Colony morphology is rough. Characteristics are unlike those of any presently defined species. The isolates produced postive niacin, catalase, and 2-week arylsulfatase reactions and were susceptible to neotetrazolium chloride (1:40,000), streptomycin (2 micrograms/ml), and rifampin (0.25 micrograms/ml). Chromogenicity, nitrate reduction, quantitative catalase, Tween hydrolysis, urease, tellurite reduction, pyrazinamidase, and 3-day arylsulfatase tests were negative, and the isolates were resistant to thiophene-2-carboxylic acid hydrazide (10 micrograms/ml) and isoniazid (10 micrograms/ml). Optimum growth in broth was determined to be in 7H9 medium with Dubos oleic albumin complex, Tween 80, and mycobactin J at 37 degrees C without CO2 or agitation and in low medium depth. This Mycobacterium sp. may be a subspecies or biovariant of Mycobacterium paratuberculosis, or it may represent a new species of Mycobacterium. It is suggested that this Mycobacterium sp. may play an etiological role in some cases of Crohn's disease.
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PMID:Characteristics of an unclassified Mycobacterium species isolated from patients with Crohn's disease. 651 78

Thirteen Campylobacter-like organisms (CLOs) isolated from rectal cultures from homosexual men were studied. Like catalase-positive Campylobacter species, CLOs were curved gram-negative rods that did not grow aerobically, were motile, were oxidase- and catalase-positive, and did not utilize glucose. However, CLOs could not be classified within any of the Campylobacter species because they grew slowly and had unusual colony morphology; did not grow at 25 C, hydrolyze hippurate, produce H2S in triple sugar-iron agar, or tolerate 2% NaCl; were inhibited by 30-micrograms disks of nalidixic acid; and tolerated 1% glycine and 0.04% triphenyltetrazolium chloride. Three groups of CLOs were identified based on differences in nitrate reduction, growth at 42 C, and sensitivity to cephalothin. By the colony hybridization technique, whole-cell DNA isolated from a strain in each CLO group hybridized with DNA from other strains in the same group, but not with strains in other groups or with reference strains of catalase-positive Campylobacter species.
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PMID:Characterization of Campylobacter-like organisms isolated from homosexual men. 669 90

Ninety-seven strains of clinically isolated Corynebacterium strains, probably identical with Corynebacterium JK, are described especially in regard to growth in relation to different lipid substances. The corynebacteria formed a homogeneous group of strict aerobic slow-growing, catalase-positive, urease-and-nitrate-negative typical coryneform rods. Acid was produced from glucose and maltose. Growth was stimulated in the presence of different lipid substances and lipodependence was suggested by satellite growth only around oleic acid drops on otherwise lipid-depleted agar plates. Generally the isolated corynebacteria were resistant to clinically achievable concentrations of penicillins, cephalosporines and aminoglucosides but uniformly sensitive to vancomycin and rifamycin.
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PMID:Multiresistant lipophilic corynebacteria from clinical specimens. Biochemical reactions and antimicrobial agents susceptibility. 671 4

Differentiation-arrested monolayer lung cell cultures were developed from day 18, 20, and 22 rat fetuses and 3-day-old neonatal rats. These cultures were examined for antioxidant enzyme activity, and the values obtained were compared with previously reported in vivo activity. All cultures were catalase deficient, and activity could be restored by the addition of 0.25 microM Fe(NO3)3 X 9H2O to the culture medium. The other measured antioxidant enzymes--copper-zinc and manganese superoxide dismutase, glutathione peroxidase, and glucose 6-phosphate dehydrogenase-demonstrate gestation-dependent increases of activity in vivo that were not evident in vitro, supporting the concept of a circulating "maturation factor" during fetal life. When cultures from fetal days 20 and 22 and from neonatal day 3 lungs were challenged with 50% oxygen in the presence of serum, antioxidant enzyme activities were unchanged, and there was no evidence of cell damage as assessed by release of lactate dehydrogenase. In the absence of serum, however, fetal day 20 (but not fetal day 22 or neonatal day 3) lung cells showed evidence of cell damage and increased antioxidant enzyme activities. It is concluded that cultured immature fetal cells are more susceptible to oxygen toxicity than those derived from mature fetal or neonatal animals. This increased susceptibility cannot be explained on the basis of the reduced antioxidant enzyme activity observed in vivo.
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PMID:Differentiation-arrested rat fetal lung in primary monolayer cell culture. III. Antioxidant enzyme activity. 674 12

The biochemical tests, namely, niacin, catalase, nitrate reduction, tween hydrolysis, tellurite reduction, arylsulfatase and urease tests were carried out for all the mycobacteria which are immunogenically closely related to M. leprae. Among them only M. vaccae shows closest relationship with M. leprae when compared with its communicated data. Except for the tellurite reduction test which was variable in case of M. leprae, all the other tests were found similar to that of M. leprae. In the next experiment, the thin-layer chromatographic pattern of mycolates from M. leprae has been compared with that of M. leprae. The presence of Keto-mycolate in the cell wall structure of both M. vaccae and M. leprae also reflects their biochemical relationship at their ultrastructural level.
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PMID:Biochemical correlation of M. vaccae with M. leprae. 675 76

The catalase activity of a non-proliferating suspension of Pseudomonas fluorescens doubled after six hours incubation in a 50 mM phosphate buffer medium (pH 7.3). The same effect was observed in a peptone medium. The increased activity was due to induced enzyme synthesis, and not to activation of preexisting catalase. Induced catalase was separated by electrophoresis from deuterium labelled constitutive catalase. The enzyme was also induced under anaerobic conditions in phosphate buffer or in culture when nitrate was supplied as an electron acceptor. Induction was considerably increased by the addition of various nucleotides and amino acids to the incubation medium.
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PMID:Inducible catalase in Pseudomonas fluorescens. 678 95

The use of eight rapid tests for the identification of 1307 strains of mycobacteria belonging to 18 species was evaluated. The standard niacin, nitrate-reductase and catalase tests were supplemented by new tests for the detection of beta glucosidase, urease, penicillinase, trehalase and cephalosporinase. This combination of eight rapid tests was not able to replace more conventional procedures but in some cases was of value in discriminating between closely related species.
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PMID:Evaluation of rapid tests for the identification of mycobacteria. 681 37

A detection procedure was developed in which a modified Lactobacilli MRS medium was used to enrich for Sporolactobacillus from a variety of foods, feed soil and environmental samples. Each sample was rinsed with 50 ml of a modified Lactobacilli MRS broth containing 1.0% (w/v) alpha-methyl glucoside 0.1% (w/v) potassium sorbate, 0.00224% (w/v) bromocresol green indicator, adjusted to pH 5.5 with acetic acid and incubated at 37 degrees C for 7 d under 5% CO2. Volumes of 2 ml from each sample were heat shocked at 80 degrees C for 5 min and 0.1 ml spread onto plates of Lactobacilli MRS agar (Difco), pH 5.5 and APT agar (BBL), pH 5.5. Plates were incubated for 5 d and suspect colonies were tested for catalase production, benzidine reaction, nitrate reduction, motility and Gram stain reaction. This method was demonstrated to be selective for Sporolactobacillus.
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PMID:Selective method for the isolation of Sporolactobacillus from food and environment sources. 685

A mycobacterial strain known as Mycobacterial strain W was analysed for its growth characteristics and biochemical traits. This strain was found to be a rapid grower, with luxurient growth on Lowenstein-Jensen medium, Dubos agar, Middlebrook's agar and Sauton's medium. Colonies were smooth, convex and nonpigmented. Some of the colonies which appeared rough were similar to smooth colonies at least in biochemical characteristics. This organism was tolerant to wide range of temperatures and to chemical substances like thiophene - carboxylic acid hydrazide, isoniazid, sodium chloride but not to bile salts. It was negative for niacin production, for various amidases, urease production, 3 day arylsulfatase test and also for Tween 80 hydrolysis. On the other hand this strain was found to be positive for semiquantitative catalase, heat resistant catalase, nitrate reduction, sodium salicylate degradation, tellurite reduction, 14 day arylsulfatase test and fermentation of fructose. This organism could utilize sodium nitrate and sodium nitrite as sources of nitrogen but didn't exhibit any utilization of fructose, arabinose as only sources of carbon. Significance of these findings is discussed.
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PMID:A report on the biochemical analysis of Mycobacterium W. 702 33

A total of 136 strains of Actinobacillus actinomycetemcomitans were studied for 135 features. All isolates were small nonmotile capnophilic gram-negative rods which grew with no requirement of X or V growth factors. They all decomposed hydrogen peroxide, were oxidase-negative and benzidine-positive, reduced nitrate, produced strong alkaline and acid phosphatases, and fermented fructose, glucose and mannose. Variable fermentation results were obtained with dextrin, maltose, mannitol and xylose. Some isolates produced small amounts of gas. Representative strains of Haemophilus aphrophilus were morphologically and biochemically quite similar to A. actinomycetemcomitans. Characters which should prove to be useful to identify and distinguish these two species include catalase reaction. fermentation of lactose, starch, sucrose and trehalose, and resistance to sodium fluoride. This information allows a rapid diagnosis by species and may be helpful in studies of infections involving these organisms.
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PMID:Salient Biochemical Characters of Actinobacillus actinomycetemcomitans. 706 13


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