Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.10.3.3 (
ascorbate oxidase
)
778
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Herein, a novel immunosensing strategy was designed for the sensitive fluorescence/visual detection of
alpha-fetoprotein
(
AFP
), by combining the enzyme-controlled formation of polydopamine (PDA) nanoparticles with the dissolution of manganese dioxide (MnO
2
) nanoflakes. Initially, a sandwich-type immunoreaction was fabricated on a capture antibody-coated microplate by using detection antibody/
ascorbate oxidase
-conjugating gold nanoparticles (AO
X
-AuNP-Ab
2
) as the detection probe. The
ascorbate oxidase
, carried within the immunocomplex, oxidized ascorbic acid (AA) to dehydroascorbic acid (DAA) to inhibit the dissolution of the MnO
2
nanoflakes. This resulted in an increment in fluorescent PDA nanoparticles from the oxidation of dopamine (DA) into its quinone derivative with the aid of the MnO
2
nanoflakes. Under optimal conditions, the fluorescence intensity of the PDA nanoparticles increased with increasing
AFP
concentration within the dynamic working range of 0.05-20 ng mL
-1
, with a detection limit (LOD) of 17.3 pg mL
-1
. The sensor also showed high specificity and acceptable accuracy for the detection of target
AFP
against other potential cancer biomarkers. Importantly, this study demonstrated for the first time that fluorescent PDA nanoparticles could be used as signal-generation tags and applied to an enzyme immunoassay. Although this methodology focused on
AFP
detection, it would be suitable for the detection of other cancer biomarkers if the corresponding antibodies were employed.
...
PMID:In situ synthesis of fluorescent polydopamine nanoparticles coupled with enzyme-controlled dissolution of MnO
2
nanoflakes for a sensitive immunoassay of cancer biomarkers. 3226 18
