Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.10.3.3 (ascorbate oxidase)
 778 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We evaluated the efficacies of five treatment procedures for eliminating ascorbate interference in the enzymatic determination of urinary oxalate. Aliquots of urine samples, containing different amounts of added ascorbate and oxalate, were individually subjected to ferric chloride, sodium nitrite, sodium periodate, charcoal, or ascorbate oxidase treatment to eliminate ascorbate interference. Oxalate contents of the urine samples were then determined by a banana oxalate oxidase-horseradish peroxidase-linked assay with 3-methyl-2-benzothiazolinone hydrazone and 3-(dimethylamino)benzoic acid as chromogens. Only those urine samples treated with ascorbate oxidase or charcoal consistently gave recovery of oxalate close to 100%. Treatment with other reagents, though improving the recovery of oxalate, gave inconsistent results. On the basis of these data, we describe procedures for simply and reliably assaying oxalate by using banana oxalate oxidase.
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PMID:Five treatment procedures evaluated for the elimination of ascorbate interference in the enzymatic determination of urinary oxalate. 204 51

A continuous flow method is described for estimation of urinary oxalate, using oxalate oxidase (EC 1.2.3.4) and ascorbate oxidase (EC 1.10.3.3) immobilised on the inner surface of O-alkylated nylon tubes. Linearity, precision, oxalate recovery, freedom from interference by other urinary substances, accuracy, specificity, absence of interaction between samples and correlation with an established enzymic method were all excellent. The method has advantages over other methods in terms of speed, ease of use and cost. As the immobilised enzyme system has been stable for 15 months the method is suitable for both research and routine use.
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PMID:Direct determination of urinary oxalate by a continuous flow method. 238 Dec 71