Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.10.3.2 (
laccase
)
4,656
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A new label--
laccase
from the fungus Coriolus hirsutus--was applied for solid-phase enzyme-linked immunosorbent assays of the pesticide 2,4-dichlorophenoxyacetic acid (
2,4-D
). Two proposed assays are based on (1) competitive binding of antibody-
laccase
conjugate with immobilized
2,4-D
-protein conjugate and
2,4-D
in tested sample, and (2) competition of
2,4-D
and
2,4-D
-
laccase
conjugate for binding with immobilized antibodies. Kinetic and concentration dependencies for these reactions were studied, and the ELISAs were optimized in accordance with the data obtained. The elaborated systems permit the detection of
2,4-D
in concentrations down to 10-20 ng/mL; time of the assays is 1.5-2 h. The main advantage of the
laccase
label, in comparison with the widely used peroxidase one, lies in the lack of hydrogen peroxide from substrate mixture, because dissolved oxygen plays the role of oxidizer.
...
PMID:Laccase from Coriolus hirsutus as alternate label for enzyme immunoassay. Determination of pesticide 2,4-dichlorophenoxyacetic acid. 1039 Aug 10
Amperometric biosensors using
laccase
from Trametes versicolor as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (
2,4-D
). Laccase enzyme was immobilized by gelatin and glutaraldehyde onto a Clark oxygen probe and screen printed electrodes (SPEs). Amperometric and chronoamperometric measurements were carried out with the biosensors. First, the effect of
laccase
activity on the biosensor performances was investigated for both biosensors, and then optimum pH and temperature and also thermal stability of the biosensors were tested. In addition, the detection ranges of some phenolic compounds were obtained by the help of calibration graphs of them. In repeatability studies, variation coefficients and standard deviations for both biosensors were also calculated by the studies done for this purposes. Finally, the biosensors were applied to the determination of
2,4-D
in a real herbicide sample.
...
PMID:Construction and comparison of Trametes versicolor laccase biosensors capable of detecting xenobiotics. 2038 Jun 15
