Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.10.3.2 (laccase)
 4,656 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To obtain a bacterial consortium that can degrade azo dyes effectively, a bacterial consortium was enriched that can degrade Metanil yellow effectively. After 6 h, 96.25% Metanil yellow was degraded under static conditions by the bacterial consortium, which was mainly composed of Pseudomonas, Lysinibacillus, Lactococcus, and Dysgonomonas. In particular, Pseudomonas played a main role in the decolorization process. Co-substrate increased the decolorization rate, and yeast powder, peptone, and urea demonstrated excellent effects. The optimal pH value and salinity for the decolorization of azo dyes is 4-7 and 1% salinity respectively. The bacterial consortium can directly degrade many azo dyes, such as direct fast black G and acid brilliant scarlet GR. Azo reductase activity, laccase activity, and lignin peroxidase activity were estimated as the key reductase for decolorization, and Metanil yellow can be degraded into less toxic degradation products through synergistic effects. The degradation pathway of Metanil yellow was analyzed by Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry, which demonstrated that Metanil yellow was cleaved at the azo bond, producing p-aminodiphenylamine and diphenylamine. These findings improved our knowledge of azo-dye-decolorizing microbial resources and provided efficient candidates for the treatment of dye-polluted wastewaters.
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PMID:Performance of a newly enriched bacterial consortium for degrading and detoxifying azo dyes. 3131 41

As a result of the use of a large amount of salt in dye industries, azo dye decolorization is often needed under hypersaline environments and low dissolved oxygen. Consortium GG-1, which is able to decolorize azo dyes in high salt concentrations and microaerophilic conditions, can be enriched using Metanil Yellow. Consortium GG-1 is mainly composed of Zobellella (62.25%), Rheinheimera (12.4%) and Marinobacterium (9.44%) and is able to decolorize azo dyes under 1%-10% salinity. The activities of azoreductase, laccase and lignin peroxidase were also measured. Together with the detected intermediates and the results obtained from FTIR, the decolorization process of Metanil Yellow was proposed. The influences of pH, initial concentration of azo dyes and concentration of yeast extract on the decolorization rate were also detected. Meanwhile, consortium GG-1 was identified with wide substrate specificity to dyes such as Direct Blue B, Acid Black ATT, and Acid Violet 7. Therefore, consortium GG-1 was identified with potential use in azo dye elimination.
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PMID:Azo dye decolorization by a halotolerant consortium under microaerophilic conditions. 3183 62