Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.10.3.2 (laccase)
 4,656 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The growth effect, tolerance and oxidative enzymes activities of Xerocomus chrysenteron under different concentrations of DDT were studied at the condition of pure culture. The changes of biomass accumulation and laccase activity were also examined along with the liquid medium period under the DDT concentration of 80.0 mg L(-1). The results show that various DDT concentrations don't change the growth mode of the studied ectomycorrhizal fungi, which are all in the mode of classic Logistic growth. Xerocomus chrysenteron has a good tolerant ability to the DDT stress, whose hemi-inhibit concentration reaches 139.75 mg L(-1). Under the liquid medium of 80.0 mg L(-1) of DDT, Xerocomus chrysenteron grows normally and after 36 days and the residue of DDT in the liquid medium is only 3.5% of the original concentration. Under the high concentration of DDT, the laccase and peroxidase activities significantly increase. The laccase is detected since the 16th day. After 36 days' culture, the laccase activity and specific activity in liquid medium reach 107.24 U L(-1) and 61.77 U g(-1) respectively. The ectomycorrhizal fungi Xerocomus chrysenteron responses to the DDT stress in various ways, which suggests large potential of biodegradation or mineralization of DDT.
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PMID:[Tolerance and enzyme response of ectomycorrhizal fungi Xerocomus chrysenteron to DDT stress]. 1864 45

This study investigated the effect of Cd on remediation of DDT contaminated soil using free laccase and immobilized reversed micelles laccase. The results show: degradation rates of ingredients of DDT and total DDT reduced with increasing of Cd concentration, the higher percentage of components in total DDT, the greater impact of Cd on them. Degradation rates of different components were p, p'-DDT > p, p'-DDD > o,p'-DDT > p, p'-DDE. When Cd concentration was 0, 0.5, 1 and 2 mg x kg(-1), the responding degradation rates of DDTs were measured at 50.68%, 32.50%, 14.92% and 13.40%, respectively. Compared with free laccase, degradation rate of total DDT by immobilized reversed micelles laccase nearly increased by 20% in soil without Cd and 30% in soil with 0.5 mg x kg(-1) Cd, implying that immobilized reversed micelles laccase is more effective for DDT degradation than free laccase.
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PMID:[Effect of Cd on remediation of DDT contaminated soil using different laccase forms]. 1883 95

High residues of DDT in agricultural soils are of concern because they present serious threats to food security and human health. This article focuses on remediation of DDT-contaminated soil using laccase under different soil oxygen and soil pH conditions. The laboratory experiment results showed significant effects of soil oxygen conditions and soil pH on remediation of DDT-contaminated soil by laccase at the end of a 25-d incubation period. This study found the positive correlation between the concentration of oxygen in soil and the degradation of DDT by laccase. The residue of DDTs in soil under the atmosphere of oxygen decreased by 28.1% compared with the atmosphere of nitrogen at the end of the incubation with laccase. A similar pattern was observed in the remediation of DDT-contaminated soil by laccase under different flooding conditions, the higher the concentrations of oxygen in soil, the lower the residues of four DDT components and DDTs in soils. The residue of DDTs in the nonflooding soil declined by 16.7% compared to the flooded soil at the end of the incubation. The residues of DDTs in soils treated with laccase were lower in the pH range 2.5-4.5.
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PMID:Effects of soil oxygen conditions and soil pH on remediation of DDT-contaminated soil by laccase from white rot fungi. 2061 49

In order to explore the microscopic degradation mechanism of organic pesticides degrading enzymes, we used molecular docking method to investigate the binding modes of DDT to laccase and chlorpyrifos to organophosphorus hydrolase, and obtained the corresponding complex structures. According to the principle of minimum scoring, the results showed that the MolDock scores were -103.134 and -111.626, re-rank scores were -72.858 and -80.261, respectively. And we used LPC/CSU server search the interactions between organic pesticides and their degrading enzymes. Our results showed that hydrophobic interaction was the strongest contacts in DDT-laccase complex, and both hydrogen bonds and hydrophobic interactions were the strongest contacts when chlorpyrifos-organophosphorus hydrolase complex. The amino acid residues Tyr224 in laccase and Arg254 in organophosphorus hydrolase were detected to play significant roles in catalytic processes.
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PMID:[Biodegradation mechanism of DDT and chlorpyrifos using molecular simulation]. 2262 2

Understanding the effects of chronic chemical contamination on natural populations of marine organisms is complex due to the combined effects of different types of pollutants and environmental parameters that can modulate the physiological responses to stress. Here, we present the effects of a chronic contamination in a marine bivalve by combining multiple approaches that provide information on individual and population health. We sampled variegated scallops (Mimachlamys varia) at sites characterized by different contaminants and contamination levels to study the short and long-term (intergenerational) responses of this species to physiological stress. We used biomarkers (SOD, MDA, GST, laccase, citrate synthase and phosphatases) as indicators of oxidative stress, immune system alteration, mitochondrial respiration and general metabolism, and measured population genetic diversity at each site. In parallel, concentration of 14 trace metals and 45 organic contaminants (PAHs, PCBs, pesticides) in tissues were measured. Scallops were collected outside and during their reproductive season to investigate temporal variability in contaminant and biomarker levels. Our analyses revealed that the levels of two biomarkers (Laccase-type phenoloxidase and malondialdehyde) were significantly correlated with Cd concentration. Additionally, we observed significant seasonal differences for four of the five biomarkers, which is likely due to the scallop reproductive status at time of sampling. As a source of concern, a location that was identified as a reference site on the basis of inorganic contaminant levels presented the same level of some persistent organic pollutants (DDT and its metabolites) than more impacted sites. Finally, potential long-term effects of heavy metal contamination were observed for variegated scallops as genetic diversity was depressed in the most polluted sites.
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PMID:Short-Term and Long-Term Biological Effects of Chronic Chemical Contamination on Natural Populations of a Marine Bivalve. 2693 82