Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.10.3.2 (laccase)
 4,656 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The degradation of textile effluent using microorganisms has been studied extensively, but disposal of generated biomass after dye degradation is a serious problem. The isolated Sphingobacterium sp. ATM was found to decolorize dye Direct Red 5B (DR5B) and simultaneously it produced polyhydroxyhexadecanoic acid (PHD). The organism decolorized DR5B at 500mgl(-1) concentration within 24h of dye addition and gave optimum production of PHD. The medium contains carbon source as a molasses which was found to be more significant within all carbon sources used. The Nuclear Magnetic Resonance spectroscopy (NMR), Fourier Transform Infrared spectroscopy (FTIR) and Gas Chromatography-Mass Spectroscopy (GC-MS) characterization of polyhydroxyalkanoates obtained revealed the compound as a polyhydroxyhexadecanoic acid. The activity of PHA synthase was found more at 24h after dye addition. The enzymes responsible for dye degradation include veratrol oxidase, laccase, DCIP (2,6-dichlorophenol-indophenol) reductase, riboflavin reductase and azo reductase was found to be induced during decolorization process. The FTIR analysis of samples before and after decolorization of dye confirmed the biotransformation of DR5B. The GC-MS analysis of product obtained led to the identification of two metabolites after biotransformation of dye as p-amino benzenesulfonic acid and naphthalene-1-ol.
 ...
PMID:Production of polyhydroxyhexadecanoic acid by using waste biomass of Sphingobacterium sp. ATM generated after degradation of textile dye Direct Red 5B. 2003 99

The degradation of textile effluent using microorganisms has been studied extensively, but disposal of generated biomass after dye degradation is a serious problem. Among all tested microorganisms, isolated Sphingobacterium sp. ATM effectively decolorized (100%) the dye Direct Blue GLL (DBGLL) and simultaneously it produced (64%) polyhydroxyhexadecanoic acid (PHD). The organism decolorized DBGLL at 300 mg l(-1) concentration within 24 h of dye addition and gave optimum production of PHD. The organism also decolorized three combinations of mixture of dyes. The organism decolorized textile effluent too when it was combined with medium. The organism produced a maximum of 66% and 61% PHD while decolorizing mixture of dyes and textile effluent respectively. Molasses was found to be more significant within all carbon sources used. The activity of polyhydroxyalkanoate (PHA) synthase was found to be higher after 24 h of addition of DBGLL. The enzymes responsible for dye degradation, viz. veratryl alcohol oxidase, laccase, DCIP (2,6-dichlorophenol-indophenol) reductase, riboflavin reductase, and azo reductase were found to be induced during decolorization process of DBGLL and mixture of dyes. There was significant reduction in chemical oxygen demand (COD) and biological oxygen demand (BOD). FTIR analysis of samples before and after decolorization of dye confirmed the biotransformation of DBGLL.
 ...
PMID:Exploring the ability of Sphingobacterium sp. ATM to degrade textile dye Direct Blue GLL, mixture of dyes and textile effluent and production of polyhydroxyhexadecanoic acid using waste biomass generated after dye degradation. 2059 65