Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.10.3.2 (laccase)
 4,656 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

cDNA clones for ascorbate oxidase were isolated from a cDNA library made from cucumber (Cucumis sativus) fruit mRNA. The library was screened with synthetic oligonucleotides that encode the NH2-terminal sequence of this enzyme. Nucleotide sequence analysis of the cloned cDNA inserts revealed a 1761-base-pair open reading frame that encoded an NH2-terminal signal peptide of 33 amino acids and a mature enzyme of 554 amino acids (Mr, 62,258). The amino acid sequence deduced from nucleotide sequence analysis agrees with the NH2-terminal amino acid sequence of the purified ascorbate oxidase, as determined by microsequencing methods. Cucumber ascorbate oxidase contained four histidine-rich regions with striking sequence homology to the corresponding parts of the other multicopper oxidases such as Neurospora crassa laccase and human ceruloplasmin and, to some extent, to a low molecular weight copper protein such as plastocyanin. Moreover, these data further support the hypothesis that the small blue copper proteins and the multicopper oxidases have evolved from the same ancestral gene. By RNA blot hybridization analysis, the mRNA for the ascorbate oxidase was found to be abundant in cucumber fruit tissue while expressed at very low levels in leaf and root tissues.
 ...
PMID:Primary structure of cucumber (Cucumis sativus) ascorbate oxidase deduced from cDNA sequence: homology with blue copper proteins and tissue-specific expression. 291 72

Fairy rings of M. oreades on pasture land were denoted by the dark-green vegetation. Grasses and rooted soils were analyzed to determine the influence of nonsymbiotic fungal mats on plant uptake of (heavy) metals. In soil colonized by M. oreades, degradation of 20-35% of plant roots in the presence of fungal laccase increased the content of dissolved organic carbon (3.74x), hexose sugar (3.75x), NH(3)/NH(4) (+) (5.1x), NO(3) (-) (11.1x), the number of aerobic bacteria (14.4x), and the formation of the phytochelators, oxalic, citric, and malonic acids. Soil pH diminished by 1.5 units mainly due to nitrification and carboxylic acid production. Although the solubility of trace elements increased (6.1x), plant roots had the same trace metal content as control plants, whereas the shoot content in Al, Cr, Fe, Ni, and Ti decreased by more than 50% due to inhibited root-to-shoot transfer. It is concluded that M. oreades and its associated bacteria increase the solubility of soil minerals significantly, but make them, due to presumed particularities in their complexation, less plant available and less re-complexable for the translocation into the plant vascular system.
 ...
PMID:Factors influencing water solubility and plant availability of mineral compounds in the tripartite fairy rings of Marasmius oreades (BOLT.: FR.) FR. 1567 62

Preparation of cellulose-polyamine composite films and beads, which provide high loading of primary amines on the surface allowing direct one-step bioconjugation of active species, is reported using an ionic liquid (IL) dissolution and regeneration process. Films and bead architectures were prepared and used as immobilization supports for laccase as a model system demonstrating the applicability of this approach. Performance of these materials, compared to commercially available products, has been assessed using millimeter-sized beads of the composites and the lipase-catalyzed transesterification of ethyl butyrate.
 ...
PMID:Ionic liquid-reconstituted cellulose composites as solid support matrices for biocatalyst immobilization. 1615 85

Although the commercially important mushroom Lentinus (= Lentinula) edodes (Berk.) Sing. can be rapidly cultivated on supplemented wood particles, fruiting is not reliable. This study addressed the problem by developing more information about growth and development on a practical oakwood-oatmeal medium. The study determined (i) the components degraded during a 150-day incubation at 22 degrees C, (ii) the apparent vegetative growth pattern, (iii) the likely growth-limiting nutrient, and (iv) assays that can be used to study key extracellular enzymes. All major components of the medium were degraded, lignin selectively so. The vegetative growth rate was most rapid during the initial 90 days, during which weight loss correlated with glucosamine accumulation (assayed after acid hydrolysis). The rate then slowed; in apparent preparation for fruiting, the cultures rapidly accumulated glucosamine (or its oligomer or polymer). Nitrogen was growth limiting. Certain enzyme activities were associated with the pattern of medium degradation, with growth, or with development. They included cellulolytic system enzymes, hemicellulases, the ligninolytic system, (gluco-)amylase, pectinase, acid protease, cell wall lytic enzymes (laminarinase, 1,4-beta-d-glucosidase, beta-N-acetyl-d-glucosaminidase, alpha-d-galactosidase, beta-d-mannosidase), acid phosphatase, and laccase. Enzyme activities over the 150-day incubation period with and without a fruiting stimulus are reported. These results provide a basis for future investigations into the physiology and biochemistry of growth and fruiting.
 ...
PMID:Extracellular Enzymes Produced by the Cultivated Mushroom Lentinus edodes during Degradation of a Lignocellulosic Medium. 1634 18

The white rot fungi Lentinula edodes, Phanerochaete chrysosporium, Pleurotus sajor-caju, Flammulina velutipes, and Schizophyllum commune were grown in liquid media containing C-lignin-labelled wood, and the formation of water-soluble C-labelled products and CO(2), the growth of the fungi, and the activities of extracellular lignin peroxidase, manganese peroxidase, and laccase were measured. Conditions that affect the rate of lignin degradation were imposed, and both long-term (0- to 16-day) and short-term (0- to 72-h) effects on the production of the two types of product and on the activities of the enzymes were monitored. The production of CO(2)-labelled products from the aqueous ones was also investigated. The short-term studies showed that the different conditions had different effects on the production of the two products and on the activities of the enzymes. Nitrogen sources inhibited the production of both products by all species when differences in growth could be discounted. Medium pH and manganese affected lignin degradation by the different species differently. With P. chrysosporium, the results were consistent, with lignin peroxidase playing a role in lignin solubilization and manganese peroxidase being important in subsequent CO(2) production.
 ...
PMID:Solubilization and mineralization of lignin by white rot fungi. 1634 81

The monoolein-based liquid crystalline cubic phase was used as the matrix to incorporate redox enzymes--glucose (GOx), pyranose (PyOx) oxidases and laccase. Thin layer of the cubic phase embedding GOx or PyOx activated glucose oxidation in the presence and absence of appropriate mediators. The electrodes exhibited unchanged voltammetric response to glucose for not less than six days. The potentials and ratio of catalytic to diffusion currents could be modified by choosing appropriate electroactive probes as mediators. Ferrocenecarboxylic acid and Ru(NH3)6(2+) provided contact between the electrode and the enzyme. The sensitivity to glucose for glucose oxidase was 0.4+/-0.05, 11+/-3.1 microA/cm2/mM without mediator and with ferrocenecarboxylic acid respectively and 0.9+/-0.06, 31+/-5.6 microA/cm2/mM for pyranose oxidase without and with mediator. The system based on glucose oxidase and Ru(NH3)6(2+) as mediator was found useful due to the most negative potential of the process. The catalyses of oxygen reduction by two laccases: Cerrena unicolor and Trametes hirsuta embedded in the cubic phase together with 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonate (ABTS) as the mediator were found efficient and the reduction potential was positive enough to be considered in the application of lyotropic liquid crystals as a material for biofuel cells.
 ...
PMID:Catalytic activity of oxidases hosted in lipidic cubic phases on electrodes. 1728 44

Extracellular laccase produced by Cerrena unicolor was immobilized by adsorption or covalent bonds formation on the cellulose-based carrier Granocel. Immobilization was optimized by changing the anchor groups and the methods of activation/immobilization. On the base of measured activity and stability of immobilized preparations, the covalent method was selected. It was shown that coupling of the enzyme to the carrier via divinyl sulfone or glutaraldehyde yielded an enzyme-carrier preparation of high activity and storage stability. Further optimization of the carrier's superstructure consisted in changing pore diameters and amount of functional groups on the carriers surface. Three-fold higher activity was noted when the enzyme was immobilized on NH2-modified Granocel with the highest size exclusion limit and amino group content. Relatively low products sorption was observed on the carrier surface. The effects of protein concentration and pH-value of the coupling mixture on immobilization efficiency were evaluated also.
 ...
PMID:Laccase immobilization on the tailored cellulose-based Granocel carriers. 1798 30

Graphite electrode modified with laccase from Cerrena unicolor served as a biosensor for detection of 30 phenolic compounds with different structures. Some correlations of the sensor response to the structures of substrates are discussed. This biosensor responded to: (i) nanomolar concentrations of some of the selected phenolic compounds, e.g., 2,6-dimethoxyphenol, coniferyl alcohol, caffeic acid, DOPAC and hydroquinone, (ii) micromolar concentrations, e.g., ferulic acid, syringic acid, dopamine, 3,4-dihydroxybenzoic acid and dl-noradrenaline, and (iii) millimolar concentrations in the case of phenol and 4-hydroxybenzaldehyde. Among the ortho- or para-substituted phenols, the sensitivity of the C. unicolor laccase-modified electrode increased in the following order H, CH(3), OH, OCH(3) and NH(3)(+) but in the case of para-substituted phenols, the K(m)(app) values were lower. The sensitivity of the laccase electrode increased with an additional OH group in para-substituted phenols. In the case of the selected compounds, kinetic data from electrochemical flow injection system were compared with those obtained from experiments in solution.
 ...
PMID:Amperometric detection of mono- and diphenols at Cerrena unicolor laccase-modified graphite electrode: correlation between sensitivity and substrate structure. 1897 Jan 11

Phlebia radiata strain BP-12-2, isolated from forest soil, decolorized fungal melanin and produced an extracellular laccase in culture. The enzyme was purified to homogeneity and characterized. It showed no absorption bands in the ultraviolet above 300 nm or visible regions. It differed from the reported laccases in substrate specificity, kinetic parameters, and NH2-terminal amino acid sequences.
 ...
PMID:Purification and characterization of an extracellular laccase from Phlebia radiata strain BP-11-2 that decolorizes fungal melanin. 1935 24

Nuclear diamination of p-hydrobenzoquinones with aromatic and aliphatic primary amines was catalysed by an immobilised commercial laccase, Denilite II Base, from Novozymes. The amine and the p-hydrobenzoquinone was reacted under mild conditions (at room temperature and at 35 degrees C) in a reaction vessel open to air in the presence of laccase and a co-solvent to afford, exclusively, the diaminated p-benzoquinone. These compounds may have potential antiallergic, antibiotic, anticancer, antifungal, antiviral and/or 5-lipoxygenase inhibiting activity.
 ...
PMID:Diamination by N-coupling using a commercial laccase. 2012 36


1 2 Next >>